Submit Manuscript | http://medcraveonline.com of tissue samples [1,2]. Rationalisation of biomarkers using the conventional histopathological procedure is time consuming, laborious and pricey especially, when several markers are to be tested on various specimens. So, TMA is expected to surmount these substantial problems [3]. Tissue microarrays are produced by harvesting cylindrical tissue core...

ARID1A is a known suppressor of tumour formation and the Human Protein Atlas antibody HPA005456 has been demonstrated in previous literature to stain tumour tissue by immunohistochemistry (IHC) in formalin-fixed paraffin embedded human tissue and human cell lines. This article details the validation of this antibody for immunohistochemistry of formalin-fixed paraffin embedded murine tissue usin...

AIMS To detect intestinal microsporidiosis in paraffin wax embedded biopsy specimens using a fluorescence technique incorporating optical brighteners. METHODS Eight HIV infected patients with confirmed intestinal microsporidiosis (six with Enterocytozoon bieneusi, one with Encephalitozoon intestinalis and one with Encephalitozoon cuniculi infection) and 10 without infection were studied. Tiss...

Method All material used was autopsy material. Lupus erythematosus was in all cases the primary cause of death. Muscle was available in fifteen cases, peripheral nerves in fifteen, and synovia in five. The tissue was embedded in paraffin blocks and serially sectioned. A minimum of 300 serial sections were cut from each block, and every tenth section was mounted and stained for study. As control...

BACKGROUND DNA extraction from paraffin wax embedded tissue requires special protocols, and most described methods report an amplification success rate of 60-80%. AIMS To propose a simple and inexpensive protocol consisting of xylene/ethanol dewaxing, followed by a kit based extraction. METHOD Xylene/ethanol dewaxing was followed by a long rehydration step and a kit based DNA extraction ste...

The analysis of antigens, enzyme histochemical markers, and DNA has become an important part of the classification of some leukemias, lymphomas, and other neoplastic diseases. Many of the relevant antigens and most of the relevant enzyme histochemical activities are destroyed and others are less than optimally preserved in tissues embedded in hot paraffin. Most enzymatic activities and antigens...

background and aims. many oral squamous cell carcinomas develop from potentially malignant disorders (pmds) which include a variety of lesions and conditions characterized by an increased risk for malignant transformation. this study evaluated the quantitative expression of egfr in normal oral mucosa, oral leukoplakia and oral submucous fibrosis to predict the malignant risk in compliance with ...

The molecular analysis of formalin-fixed and paraffin-embedded tissues has application in both investigative studies and clinical practice (1). It is now possible to analyze gene expression in formalin-fixed and paraffin-embedded tissues by in situ reverse transcriptase-polymerase chain reaction (in situ RT-PCR) (2). Degradation of RNA, however, can occur at various stages of tissue preparation...

Molecular diagnostics in personalized medicine increasingly relies on the combination of a variety of analytical technologies to characterize individual diseases and to select patients for targeted therapies. The gold standard for tissue-based diagnostics is fixation in formalin and embedding in paraffin, which results in excellent preservation of morphology but negatively impacts on a variety ...

congenital toxoplasmosis is associated with variable complications including encephalitis, microcephaly, hydrocephaly, hepatitis, lymphadenopathy and even intrauterine death. presence of toxoplasma gondii in human placenta may induce congenital infection. the aim of this study was to determine the genotypes of toxoplasma gondii infection in human spontaneous aborted fetuses in shiraz, south of ...