Background Environmental and cellular inappropriate conditions can cause damages to cells nucleotide poll. Deamination and oxidation damages interfere with cell�s vital reactions. Inosine triphosphate pyrophosphatase (ITPA), an evolutionary conserved enzyme, plays a critical role in elimination of non-canonical bases. In human genome, the ITPA gene is located on chromosome 20 short arm and tran...

the aim of this study was to detect the glutathione s-transferase(gst) enzyme activity of healthy / cystic liver as a diagnostic biomarker for hydatidosis. in order to compare with liver tissue, the level of the gsts enzyme activity of parasite was also determined.parasites were collected from sheep liver tissue with hydatid cysts at a local abattoir and washed with pbs buffer. collected parasi...

Background and Aims: Since the produced recombinant proteins by molecular genetics techniques commonly have some limitations in the application, chimeric protein are introduced. Chimeric proteins have found widespread application for the study of protein folding, structure stability, function and immunogenisity. Methods: According to the known Immunomodulatory effect and structure of HSP70 mole...

  Objective(s):  Infection by high-risk papillomavirus is regarded as the major risk factor in the development of cervical cancer. Recombinant DNA technology allows expression of the L1 major capsid protein of HPV in different expression systems, which has intrinsic capacity to self-assemble into viral-like particles (VLP). VLPS are non-infectious, highly immunogenic and can elicit neutralizing...

introduction: toxoplasma gondii is a protozoan parasite which is globally prevalent in human and animals. toxoplasma infection is commonly asymptomatic, but can cause serious medical problems in immunocompromised individuals and in fetus. recombinant antigens of the parasite may be helpful in diagnosing the infection more precisely. the goal of this study was to construct and evaluate the funct...

Background: Erythrocytes require an ability to deform and to withstand shear stress while negotiating the microcirculation. These properties are largely due to their excess surface area per volume and the characteristics of the membrane’s protein. Deficiencies of these proteins are associated with chronic haemolysis. Methods: This was a cross sectional  study aimed at determining the prevalenc...