The phagocyte NADPH oxidase, dormant in resting cells, is activated during phagocytosis to produce superoxide, a precursor of microbicidal oxidants. The activated oxidase is a complex of membrane-integrated cytochrome b558, composed of 91-kDa (gp91phox) and 22-kDa (p22phox) subunits, and two cytosolic factors (p47phox and p67phox), each containing two Src homology 3 (SH3) domains. Here we show ...

Starting with a mutation impacting photoreceptor morphogenesis, we identify here a Drosophila gene, eyes closed (eyc), as a fly homolog of p47, a protein co-factor of the p97 ATPase implicated in membrane fusion. Temporal misexpression of Eyc during rhabdomere extension early in pupal life results in inappropriate retention of normally transient adhesions between developing rhabdomeres. Later E...

n page 1133, Bao et al. demonstrate that a neuronal growth factor signals to neighboring cells while also communicating back to the nucleus of its own cell. This two-way signaling is necessary to maintain neuronal survival. In the forward direction , either a soluble or membrane-bound form of the Nrg-1 growth factor activates erbB receptor tyrosine kinases on a variety of adjacent cells. The Nr...

n page 1133, Bao et al. demonstrate that a neuronal growth factor signals to neighboring cells while also communicating back to the nucleus of its own cell. This two-way signaling is necessary to maintain neuronal survival. In the forward direction , either a soluble or membrane-bound form of the Nrg-1 growth factor activates erbB receptor tyrosine kinases on a variety of adjacent cells. The Nr...

Recently, we showed that cultured guinea pig gastric pit cells possess a phagocyte NADPH oxidase-like activity, which was up-regulated by Helicobacter pylori lipopolysaccharide. We demonstrate here that these cells express all of the phagocyte NADPH oxidase components (gp91-, p22-, p67-, p47-, and p40-phoxes). Treatment with lipopolysaccharide increased the expression of gp91-, p22-, and p67-ph...

In HL-60 and ML-3 human myeloid cell lines, gamma-interferon (IFN-gamma) and/or tumor necrosis factor (TNF) induce synergistic accumulation of transcripts of the genes encoding the heavy chain (gp91-phox) of cytochrome b558 and the cytosolic factors p47-phox and p67-phox, components of the superoxide-generating NADPH oxidase system. The accumulation of transcripts for gp91-phox and p47-phox, as...