Coxiella burnetii and Legionella pneumophila are evolutionarily related pathogens with different intracellular infection strategies. C. burnetii persists within and is transmitted by mammalian hosts, whereas, L. pneumophila is found primarily in the environment associated with protozoan hosts. Although a type IV secretion system encoded by the defect in organelle trafficking (dot) and intracell...

Procaspase-3 (p32) is processed by upstream caspases to p12 and p20 subunits, which heterodimerize. Concomitant with formation of the active heterotetramer, p20 is autoprocessed to p17. Treatment of HL-60 cells with lactacystin, a selective inhibitor of the proteasome, exponentially increased caspase-3-like hydrolytic activity and induced apoptosis but had little or no effect on the activity of...

Unactivated human blood monocytes and monocytic THP-1 cells were found to respond to some leukemia cells by tumor necrosis factor (TNF) production. The TNF production by THP-1 cells in response to K562 cells was preceded by a rapid rise in [Ca2+]i, initiated within 1 h and terminated within 4 h as a refractory state took over. Neither the amount nor the duration of TNF production was enhanced b...

Brown and associates (2, 9) conclusively demon strated that isotopically labeled adenine was in corporated into the purines of the nucleic acids of normal tissues. The results of these and other in vestigators indicated that the uptake of the labeled compound into the cytoplasmic ribonucleic acid (RNA) was considerably greater than that into the desoxyribonucleic acid (DNA). In re generating li...

Polyclonal sera obtained from African children with acute measles were used to screen a panel of 15-mer overlapping peptides representing the sequence of measles virus (MV) fusion (F) protein. An immunodominant antigenic region from the F protein (p32; amino acids 388 to 402) was found to represent an amino acid sequence within the highly conserved cysteine-rich domain of the F protein of param...

Nematophagous fungi Pochonia chlamydosporia and P. rubescens colonize endophytically barley roots. During nematode infection, serine proteases are secreted. We have investigated whether such proteases are also produced during root colonization. Polyclonal antibodies against serine protease P32 of P. rubescens cross-reacted with a related protease (VCP1) of P. chlamydosporia, but not with barley...

increases over controls were found when root segments were incubated in phosphorus solutions containing 10-3 A D)NP ancd at lowx temlperatture. The increased Pi in Al-roots did niot result in an increase in the total amounit of l)hosphorus incorporated into phosphorylated compounds. Aluminum treatnment miiarkedly decreased the incorporation of P32 into sigar phosl)hates but increased the pool s...

Neither the P32-phosphate nor the S35-sulfate were metabolized by intact seeds until after radicle protrusion (15 to 18 hours after beginning of imbibition). Punctured seeds, however, metabolized the radioactive phosphate or sulfate within 3 hours after the beginning of imbibition. We concluded that the coats surrounding the embryo are impermeable to phosphate and sulfate ions. Most of the orga...