In order to have a better understanding of the clinical significance of Pseudomonas aeruginosa, circulating and secretory antibodies were measured. Of 100 patients diagnosed as having cystic fibrosis (CF) and an atypical mucoid P. aeruginosa cultured from their sputum, each possessed serum precipitins. These immunoprecipitates, however, were not detected in the sera of 40 CF patients, some of w...

PURPOSE To determine the genetic diversity of MDR P. aeruginosa strains isolated from burn and wound infections in Ahvaz, Iran, by ERIC-PCR. METHODS From total 99 strains of P. aeruginosa defined as MDR by using drug susceptibility testing, 66 were subjected to ERIC-PCR analysis, comprises 53 strains isolated from burn infection, and 13 randomly selected strains from wound infection with high...

conclusions this blis with broad-spectrum activity may be a potential agent for the treatment or control of drug-resistant strains of p. aeruginosa infection. results among the 96 isolates of p. aeruginosa, 2 (2.1%), 94 (97.9%), and 63 (65.6%) were non-mdr, mdr, and xdr, respectively, and 1 (1.1%) was pdr. the most effective antibiotics against these isolates were polymyxins and fosfomycin. a b...

background and objective: metallo-beta-lactamase (mbl)-mediated resistance is an emerging threat in hospital isolates of pseudomonas aeruginosa. there is not enough information from iran regarding the prevalence and the screening methods for such enzymes. the present study was undertaken to detect metallo betalactamase in strains of p. aeruginosa isolated from burned patientusingphenotypic meth...

The aim of this study was to evaluate the in vitro combination effects of aztreonam (AZT) and aminoglycosides against multidrug-resistant (MDR) Pseudomonas aeruginosa strains in Japan. We investigated 47 MDR P. aeruginosa strains collected from 8 facilities. We selected the aminoglycosides amikacin (AMK), gentamicin (GM), and arbekacin (ABK) to examine their effects when combined with AZT u...

BACKGROUND Molecular typing is integral for identifying Pseudomonas aeruginosa strains that may be shared between patients with cystic fibrosis (CF). We conducted a side-by-side comparison of two P. aeruginosa genotyping methods utilising informative-single nucleotide polymorphism (SNP) methods; one targeting 10 P. aeruginosa SNPs and using real-time polymerase chain reaction technology (HRM10S...

PURPOSE To determine the importance of Pseudomonas aeruginosa exotoxin A (ETA) as a virulence factor in corneal disease. METHODS Isogenic mutants deficient in ETA were constructed in P. aeruginosa strains PAO1 and ATCC 19660 by allelic exchange and then evaluated for virulence in a mouse model of bacterial keratitis. The effect of ETA on adherence to scarified corneal epithelium was assessed ...

Chronic Pseudomonas aeruginosa infection in cystic fibrosis (CF) leads to a damaging host inflammatory response. There are an increasing number of reports of P. aeruginosa cross-infection at CF centres. The clinical significance of acquisition of a transmissible strain for patients who already harbour P. aeruginosa is unclear. In this study, levels of inflammatory markers in clinically stable a...

The protective activity against experimental Pseudomonas aeruginosa infection of a human monoclonal antibody, MH-4H7, which is thought to recognize L-rhamnose and its neighboring residues in the outer core region of P. aeruginosa lipopolysaccharide and which binds to strains of Homma serotypes A, F, G, H, K, and M, was studied in normal, burned, and leukopenic mice. MH-4H7 at doses of 0.1 to 1....

BACKGROUND Microorganisms are characterized by two types of resistance innate and acquired. Innate resistance is associated with the construction of the surface structures. Wide use of active substances as antimicrobial compounds, especially in inhibitory concentrations, may promote the acquisition of bacterial resistance to these substances in the process of adaptation. OBJECTIVE The aim of ...