Oviduct epithelial cells are important for the nourishment and survival of ovulated oocytes and early embryos, and they respond to the steroid hormones estrogen and progesterone. Endocrine-disrupting polyhalogenated aromatic hydrocarbons (PHAH) are environmental toxins that act in part through the ligand-activated transcription factor arylhydrocarbon receptor (AhR; dioxin receptor), and exposur...

In mammals, only a few spermatozoa arrive at the fertilization site. During the last step in the journey to the egg, apart from their self-propulsion, spermatozoa may be assisted by oviduct movement and/or a guidance mechanism. The proportion of rabbit spermatozoa that arrive at the fertilization site was determined under in vivo conditions, in which either the ovulation products (secreting che...

Incubation of hen oviduct membranes with UDP-[14C]N-acetylglucosamine (GlcNAc) leads to the formation of four endogenously labeled lipids. One of these lipids has not previously been characterized. Based on its chromatographic behavior on SG-81 paper and DEAE-cellulose, it is a monophosphate diester. The oviduct lipid is extremely sensitive to mild acid hydrolysis and, based on a comparison wit...

The female reproductive organs of Sarcophaga ruficornis F. (Diptera: Sarcophagidae) consists of paired ovaries, lateral oviducts, a common oviduct, three spermathecae and paired accessory glands. Each ovary is formed of about 45-53 polytrophic ovarioles. The epithelium of lateral oviduct is formed of syncytial cells overlaid by longitudinal and circular muscle fibers. The common oviduct has syn...

We successfully replaced the ovalbumin gene of a magnum region in chickens with a human plasminogen activator. We constructed pL-eGFP, pL-tPAGFP and pL-2.8OVtPAGFP vectors and cultured 293FT chicken embryo fibroblasts, chicken primordial germ cells, Hela C127 cells, and oviduct epithelial cells. All vectors were expressed in the transfected cells, except pL-2.8OVtPAGFP vector, which was only ex...

The Chinese brown frog (Rana dybowskii) is a special amphibian with one unique physiological phenomenon, which is that its oviduct expands prior to hibernation, instead of during the breeding period. In this study, we investigate the localization and expression level of PPARγ2, leptin and leptin receptor proteins in oviduct of Rana dybowskii during  breeding period and pre-hibernation. There we...

Cladribine is a purine nucleoside analog which initiates the apoptotic mechanism within cells. Moreover, the available data confirms that cladribine, with the participation of the p53 protein, as well as the proapoptotic proteins from the Bcl-2 family, also induces the activation of the intrinsic apoptosis pathway. However, while there has been a lot of research devoted to the effect of cladrib...

The aim of this study was to investigate the immunolocalization and the existence of thyroid hormone receptors (THR) (alpha1/alpha2) in rat uterus and oviduct. For this purpose 6 female Wistar albino rats found in estrous period were used. Tissue samples fixed in 10% neutral formalin were examined immunohistochemically. Sections were incubated with primary mouse-monoclonal THR (alpha1/alpha2) a...

Up to the present time the function and significance of the zona pellucida in the development of mammalian eggs has not been fully explained. Zona-free mouse eggs will develop in vitro from the 2-cell stage, or later, up to the blastocyst stage (Tarkowski, 1961; Mintz, 1962; Gwatkin, 1963). Single blastomeres isolated at the 2-cell (Mulnard, 1965), 4and 8-cell stage (Tarkowski & Wróblewska, 196...

A study was conducted comparing ovary and oviduct development following photostimulation in two lines of turkey breeder stocks (female line and male line). Birds were euthanatized for assessment of reproductive organ morphology at 3-d intervals following photostimulation (203 d of age) to 245 d and on the day following their first oviposition. The age at first oviposition was similar for both l...