نتایج جستجو برای: osteogenic differentiation

تعداد نتایج: 227817  

2017
Kulisara Marupanthorn Chairat Tantrawatpan Pakpoom Kheolamai Duangrat Tantikanlayaporn Sirikul Manochantr

Mesenchymal stromal cells (MSCs) are multipotent cells that can give rise to different cell types of the mesodermal lineages. They are powerful sources for cell therapy in regenerative medicine as they can be isolated from various tissues, and can be expanded and induced to differentiate into multiple lineages. Recently, the umbilical cord has been suggested as an alternative source of MSCs. Al...

Journal: :Bioscience trends 2016
Nobuaki Ozeki Makio Mogi Naoko Hase Taiki Hiyama Hideyuki Yamaguchi Rie Kawai Kazuhiko Nakata

Inorganic polyphosphate [Poly(P)] induces differentiation of osteoblastic cells. In this study, matrix metalloproteinase (MMP)-13 small interfering RNA (siRNA) was transfected into human adipose tissue-derived mesenchymal stem cells (hAT-MSC) to investigate whether MMP-13 activity induced by Poly(P) is associated with osteogenic differentiation. Real-time quantitative polymerase chain reaction,...

2014
Nicola Antonio Martino Stephan Joel Reshkin Elena Ciani Maria Elena Dell'Aquila

BACKGROUND Umbilical cord matrix mesenchymal stem cells (UCM-MSCs) present a wide range of potential therapeutical applications. The extracellular calcium-sensing receptor (CaSR) regulates physiological and pathological processes. We investigated, in a large animal model, the involvement of CaSR in triggering osteogenic and neurogenic differentiation of two size-sieved UCM-MSC lines, by using A...

2016
Xiaochen Qu Zhongqiang Chen Dongwei Fan Chuiguo Sun Yan Zeng

Ossification of the ligamentum flavum (OLF) is a disorder of heterotopic ossification of spinal ligaments and is the main cause of thoracic spinal canal stenosis. Previous studies suggested that miR-132-3p negatively regulates osteoblast differentiation. However, whether miR-132-3p is involved in the process of OLF has not been investigated. In this study, we investigated the effect of miR-132-...

2013
Zhifei Zhou Bei Li Zhiwei Dong Fen Liu Yu Zhang Yang Yu Fengqing Shang Lizheng Wu Xiaojing Wang Yan Jin

AIMS Cigarette smoking is one of the high risk factors of adult chronic periodontitis and nicotine is the well established toxic substance in cigarette. However, the mechanism of nicotine induced periodontitis is still unknown. Here we studied whether nicotine impaired the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) through activating α7 nicotinic acetylcholine...

2017
Junyi Liao Xinyi Yu Xue Hu Jiaming Fan Jing Wang Zhicai Zhang Chen Zhao Zongyue Zeng Yi Shu Ruyi Zhang Shujuan Yan Yasha Li Wenwen Zhang Jing Cui Chao Ma Li Li Yichun Yu Tingting Wu Xingye Wu Jiayan Lei Jia Wang Chao Yang Ke Wu Ying Wu Jun Tang Bai-Cheng He Zhong-Liang Deng Hue H. Luu Rex C. Haydon Russell R. Reid Michael J. Lee Jennifer Moriatis Wolf Wei Huang Tong-Chuan He

Mesenchymal stem cells (MSCs) are multipotent progenitor cells that can undergo self-renewal and differentiate into multiple lineages. Osteogenic differentiation from MSCs is a well-orchestrated process and regulated by multiple signaling pathways. We previously demonstrated that BMP9 is one of the most potent osteogenic factors. However, molecular mechanism through which BMP9 governs osteoblas...

Journal: :Arteriosclerosis, thrombosis, and vascular biology 2011
Jill Wylie-Sears Elena Aikawa Robert A Levine Jeong-Hee Yang Joyce Bischoff

OBJECTIVE Cardiac valvular endothelium is unique in its ability to undergo endothelial-to-mesenchymal transformation, a differentiation process that is essential for valve development and has been proposed as mechanism for replenishing the interstitial cells of mature valves. We hypothesized that the valvular endothelium contains endothelial cells that are direct precursors to osteoblastic valv...

2015

Reference 1. Linero I, Chaparro O (2014) Paracrine Effect of Mesenchymal Stem Cells Derived from Human Adipose Tissue in Bone Regeneration. PLoS ONE 9(9): e107001. doi:10.1371/journal.pone.0107001 PMID: 25198551 Fig 3. Ad-MSC characterization. A. Osteogenic differentiation of Ad-MSC. Osteogenic differentiation was evidenced by the detection of calcium deposits with Alizarin Red staining. a. Con...

Mohammadreza Talebi Ardakani, Alireza Rafiei, Behzad Houshmand, Mohammadali Rouzegar, Mohammadreza Abrishami, Omolbanin Amjadi,

Background: Periodontal ligament stem cells (PDLSCs) are considered as a type of mesenchymal stem cell that is beneficial target for numerous clinical applications in periodontal tissue regeneration therapy. Materials and Methods: This study examined the effects of dexamethasone (Dex) on human PDLSCs in vitro. PDLSCs obtained from the roots of patient’s teeth were cultured with Dex (0....

2015
Wataru Katagiri Masashi Osugi Takamasa Kawai Hideharu Hibi Wilhelm Bloch

Secreted frizzled-related protein (sFRP)-3 is a negative regulator of Wnt signaling in human mesenchymal stem cells (hMSCs). The present study investigated the effects sFRP-3 on osteogenic differentiation by assessing osteogenic gene expression in hMSCs in vitro and by examining bone regeneration in a rat bone defect model. sFRP-3 treatment induced osteogenic differentiation in hMSCs as determi...

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