Optical coherence tomography (OCT) is a well-established optical imaging technique with micrometer-scale resolution. OCT is based on low-coherence interferometry to measure the amplitude of light backscattered by the sample being imaged [1-4]. The most significant impact of OCT is in ophthalmology for in situ examination of the pathologic changes of the retina [5-8] and measurement of the dimen...

We have developed a common-path multimodal optical microscopy system that is capable of using a single optical source and a single camera to image amplitude, phase, and fluorescence features of a biological specimen. This is achieved by varying either contrast enhancement filters at the Fourier plane and/or neutral density/fluorescence filters in front of the CCD camera. The feasibility of the ...

An average human eye can see details down to 0,07 mm in size. The ability to see smaller details of the matter is correlated with the development of the science and the comprehension of the nature. Today's science needs eyes for the nano-world. Examples are easily found in biology and medical sciences. There is a great need to determine shape, size, chemical composition, molecular structure and...

Three-dimensional (3D) reconstruction of anatomical structures can give additional insight into the morphology and function of these structures. We compare 3D reconstructions of the guinea pig inner ear, using light microscopy and orthogonal plane fluorescence optical sectioning microscopy. Applications of 3D reconstruction of the inner ear are further explored. For each method two bullas were ...

Cobalt (Co) doped Zinc Oxide (ZnO) thin films, containing different amountof Cobalt nanoparticles as the Co doping source, deposited by the sol–gel spin coatingmethod onto glass via annealing temperature at 400˚C, have been investigated by opticalcharacterization method. The effect of Co incorporation on the surface morphology wasclearly observed from scanning electron microscopy (SEM) images. ...

Recently developed optical-resolution photoacoustic microscopy (OR-PAM), which is based on the detection of optical absorption contrast, is complementary to other optical microscopy modalities such as optical confocal microscopy, optical coherence tomography, and multiphoton microscopy. A hybrid optical-mechanical scanning configuration increases the imaging speed of OR-PAM significantly, facil...

Spectrally encoded confocal microscopy and optical frequency domain imaging are two non-contact optical imaging technologies that provide images of tissue cellular and architectural morphology, which are both used for histopathological diagnosis. Although spectrally encoded confocal microscopy has better transverse resolution than optical frequency domain imaging, optical frequency domain imagi...

Laser Scanning Microscopy Techniques such as Confocal Microscopy, Two Photon Excitation Microscopy, Second Harmonic Generation Microscopy or Fluorescence Lifetime Imaging have been successfully employed to date for imaging skin cancer. However, the resolution that such techniques can provide is limited by diffraction, which prevents accurately imaging (and differentiating) optical details sized...