Caspian kutum fish (Rutilus frisii) wild broodstocks were caught by gillnet during their upstream migration and subjected to short-time confinement stress and their physiological responses have been examined. Thirty fish (average weight of 1217.6 g) were divided into three experimental groups CO (control), OV (ovaprim) and CO+OV (cortisol+ovaprim) and placed into rearing tanks (10 fish tank-1; ...

Although oocytes of many teleost fish, especially marine species, are subjected to a hydration process during meiotic maturation, which leads to an important volume increase, no noticeable hydration of the preovulatory oocyte has ever been reported in rainbow trout (Oncorhynchus mykiss). In the present study, oocyte water content and dry mass were monitored using consecutive samples taken in vi...

BACKGROUND MicroRNA (miRNA) are small non-coding RNA molecules critical for regulating cellular function, and are abundant in the maturing oocyte and developing embryo. MiRNA-21 (MIR21) has been shown to elicit posttranscriptional gene regulation in several tissues associated with rapid cell proliferation in addition to demonstrating anti-apoptotic features through interactions with PDCD4 mRNA ...

cAMP plays a critical role in the control of oocyte maturation, as a high level of cAMP maintains oocyte arrest at the first meiotic prophase. Yet this study shows that pulsing meiotically arrested denuded oocytes (DO) with cAMP induces oocyte maturation through the activation of AMP-activated protein kinase (PRKA). Short-term (3 h) pulsing of meiotically arrested oocytes with forskolin, an ade...

Currently, it is believed that toad oocyte maturation is dependent on the physiological conditions of winter hibernation. Previous antibody-blocking experiments have demonstrated that toad ubiquitin carboxyl-terminal hydrolase L1 (tUCHL1) is necessary for germinal vesicle breakdown during toad oocyte maturation. In this paper, we first supply evidence that tUCHL1 is highly evolutionarily conser...

objectives: it was aimed to investigate the effects of different doses of follicle stimulating hormone (fsh) and activin a on the growth and maturation of preantral mouse follicles during the in vitro culture. methods: preantral follicles (90-100 µm in diameter) were harvested from 6-8 week-old syrian mice and cultured in tcm199 culture medium for 6 days to see the effect of fsh and activin a. ...

We sought to investigate whether miR-378 plays a role in cumulus cells and whether the manipulation of miRNA levels in cumulus cells influences oocyte maturation in vitro. Cumulus-oocyte complexes (COCs) from ovarian follicles had significantly lower levels of precursor and mature miR-378 in cumulus cells surrounding metaphase II (MII) oocytes than cumulus cells surrounding germinal vesicle (GV...

The ultrastructural analysis of oocytes and ovarian follicles has been used to evaluate the effects of assisted reproductive techniques, such as cryopreservation or in vitro oocyte maturation. It also benefits the understanding of such complex mechanisms that occur during folliculogenesis. From the beginning of primordial follicles growth until oocyte maturation in preovulatory follicles oocyte...

Background: Intracytoplasmic sperm injection (ICSI) has become a useful technology to produce foals when availability of semen is limited or when in-vitro fertilization is desired, as is the need for subfertile mares. However, its application into clinical practice is challenging. The purpose of this review was to discuss some fundamental molecular aspects of oocyte maturation that should be co...

Oocyte developmental competence is one of the determining factors that in?uence outcomes an IVF cycle regarding ability a female gamete to reach maturation and be fertilized. A non-invasive approach using proteomic analysis oocyte cumulus cells (CCs) can used as method predict viability could potentially function molecular predictors for program prognosis. Our study was aimed at quantifying pro...