نتایج جستجو برای: one primer pair as1iiamyc5r

تعداد نتایج: 2110471  

Journal: :حفاظت گیاهان 0
سید رضا میررحیمی بیداخویدی ثمین حسینی سید احمد حسینی سید علیرضا اسمعیل زاده حسینی الهام محمدی

tomato is one of the most important vegetables in iran and throughout the world with crop loss caused by several viruses. this survey was carried out to detect the viruses causing mosaic symptoms on tomato in yazd city (iran). a total of 451 samples of tomato leaves showing mosaic symptoms were collected from fields and greenhouses during 2012-2013 growing season. collected samples were analyze...

Journal: :Journal of clinical microbiology 2000
P W Whitby K B Carter K L Hatter J J LiPuma T L Stull

Definitive identification of the species in the Burkholderia cepacia complex by routine clinical microbiology methods is difficult. Phenotypic tests to identify B. multivorans and B. vietnamiensis have been established; more recent work indicates B. stabilis may also be identified by growth characteristics and biochemical tests. However, attempts to identify genomovars I and III have, thus far,...

2007
Wayne MYRIE Nigel HARRISON Michel DOLLET Basil BEEN

Tissue samples were collected from coconut palms with symptoms of lethal yellowing (LY) disease in Jamaica. Palms were sampled at 50 sites exhibiting varying levels of mortality due to LY. Samples were assayed for phytoplasma infection by polymerase chain reaction (PCR) assays. Detected phytoplasmas were characterized by dot blot nucleic acid hybridisation, restriction fragment length polymorph...

2013
Sangeeta Kadyan Manju Panghal Khushboo Singh Jaya Parkash Yadav

Restriction fragment length analysis of 16S rRNA gene of 52 different aerobic endospore forming Bacilli (AEFB) strains with HaeIII enzyme has revealed the presence of a 460 bp long fragment in 50 AEFB strains. BLAST analysis revealed that the fragment was 463 bp long and it was located at 3' end of 16S rRNA gene. Further specificity of this fragment for AEFB strains was checked by PCR and in si...

2016
Daisuke Kurose Naruto Furuya Tetsuya Saeki Kenichi Tsuchiya Seiya Tsushima Marion K. Seier

The ascomycete fungus Mycosphaerella polygoni-cuspidati has been undergoing evaluation as a potential classical biological control agent for the invasive weed Fallopia japonica (Japanese knotweed), which has become troublesome in Europe and North America. In advance of the potential release of a biocontrol agent into a new environment, it is crucial to develop an effective monitoring system to ...

Journal: :caspian journal of internal medicine 0
massoud hajia health reference laboratories research center, ministry of health & medical education, tehran, iran. mohamad rahbar health reference laboratories research center, ministry of health & medical education, tehran, iran. marjan rahnami farzami health reference laboratories research center, ministry of health & medical education, tehran, iran. alireza dolatyar health reference laboratories research center, ministry of health & medical education, tehran, iran. mohsen imani health reference laboratories research center, ministry of health & medical education, tehran, iran. roghieh saburian health reference laboratories research center, ministry of health & medical education, tehran, iran.

background: identifying regional types and evaluating the frequency of pneumococcal strains has become increasingly important especially in vaccination. the purpose of this study was the identification and frequency determination of our regional serotype and evaluation of the performance of recent type specific multiplex pcr for the diagnosis of streptococcus pneumonia serotypes. methods: all i...

Journal: :The Journal of biological chemistry 1990
H E Huber C C Richardson

We have analyzed the processing of the RNA primer for (+) strand DNA synthesis by reverse transcriptase of the human immunodeficiency virus 1. To test for specific RNA cleavage and primer usage, we constructed a 99-base pair RNA-DNA hybrid containing the viral polypurine tract and flanking viral sequences. Although the RNase H activity of reverse transcriptase cleaves the RNA strand into multip...

Journal: :Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan 2012
Yasuyuki Matsuoka Shin-Ichiro Arami Megumi Sato Hiroyuki Haraguchi Youichi Kurimoto Shinjiro Imai Keiko Tanaka Junichi Mano Satoshi Furui Kazumi Kitta

A PCR-based method was developed to distinguish between durum/common wheat and common wheat by leveraging slight differences of DNA sequence in Starch Synthase II (SS II) coded on wheat A, B and D genomes. A primer pair, SS II ex7-U/L, was designed to hybridize with a conserved DNA sequence region found in SS II-A, B and D genes. Another primer pair, SS II-D 1769U/1889L, was constructed to reco...

Journal: :Applied and environmental microbiology 1998
K Reilly G T Attwood

A competitive PCR technique was used to enumerate the proteolytic bacterium Clostridium proteoclasticum from the rumen. A PCR primer, which circumscribes this organism and several closely related strains, was designed for a variable region within their 16S rRNA genes and was used in conjunction with a universal forward primer. This primer pair was tested for specificity against 85 ruminal bacte...

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