Anaerobic long-chain fatty acid (LCFA)-degrading bacteria were identified by combining selective enrichment studies with molecular approaches. Two distinct enrichment cultures growing on unsaturated and saturated LCFAs were obtained by successive transfers in medium containing oleate and palmitate, respectively, as the sole carbon and energy sources. Changes in the microbial composition during ...

The potential for improving long-chain fatty acids (LCFA) conversion to methane was evaluated by bioaugmenting a non-acclimated anaerobic granular sludge with Syntrophomonas zehnderi. Batch bioaugmentation assays were performed with and without the solid microcarrier sepiolite, using 1 mM oleate as sole carbon and energy source. When S. zehnderi was added to the anaerobic sludge methane product...

Addition of lard or sodium oleate to the medium used for lipase production by Pseudomonas fragi resulted in a decreased accumulation of lipase in the culture supernatant fluid without affecting cell growth. The production and activity of lipase was inhibited by lard, sodium oleate, and the salts of other unsaturated fatty acids. Some divalent cations, Tweens, lecithin, and bovine serum prevente...

The primary determinants of hepatic uptake of long chain fatty acids have been considered to be the plasma concentrations of fatty acid and albumin, with little or no intrinsic control by the hepatocyte itself. However, recent studies of liver cell suspensions have shown that in immature, adult, castrated, and hormone-treated rats, sex steroids exert striking effects on [(14)C]oleate uptake and...

The miscibility of triolein and cholesteryl oleate with 1-palmitoyl-2-oleoyl phosphatidylcholine was studied at the argon-buffer interface. The surface phase behavior of the system was analogous to that for cholesteryl ester-phospholipid mixtures in that both monolayer and double layer surface phases were formed. By considering the bulk properties of cholesteryl oleatetriolein mixtures and the ...