BACKGROUND The increase in recent outbreaks and unpredictable changes of highly pathogenic avian influenza (HPAI) H5N1 in birds and humans highlights the urgent need to develop a cross-protective H5N1 vaccine. We here report our development of a multiple-clade H5N1 influenza vaccine tested for immunogenicity and efficacy to confer cross-protection in an animal model. METHODOLOGY/PRINCIPAL FIN...

Obesity is characterized by chronic inflammation associated with neutrophil and M1 macrophage infiltration into white adipose tissue. However, the mechanisms underlying this process remain largely unknown. Based on the ability of oil-based adjuvants to induce immune responses, we hypothesized that endogenous oils derived from necrotic adipocytes may function as an immunological "danger signal."...

Traditionally, protein Ags have been injected in CFA (oil with inactivated mycobacteria) to induce immunity and with IFA (oil alone) to induce tolerance. We report here that injection of hen eggwhite lysozyme, a prototypic Ag, in CFA-induced and IFA-induced pools of hen eggwhite lysozyme-specific memory T cells of comparable fine specificity, clonal size, and avidity spectrum, but with type-1 a...

Environmental risks resulting from increasing application of herbicides have raised many concerns about human health and safety of agro ecosystems and environment. Hence, it is very important to find practical and effective methods for reducing the use of pesticides in environment as well as the increasing the efficiency of these compounds. Addition of adjuvants to spray solutions could be cons...

The vaccination of pigs with inactivated antigen requires adjuvanted vaccines. In general, two kinds of adjuvant are used to enhance the immunogenic properties of an antigen: aluminium salts or oil-based formulas [1, 9]. Aluminium salts often fail to induce a long-term response or a cellular immune response [7]. Emulsified vaccines are frequently used in pigs as they induce a long-lasting immun...

An adjuvant is any material added to a herbicide spray solution to enhance or modify the performance of the solution. Adjuvants can be divided into two general categories: spray modifiers and activators (Kirkwood, 1994). Spray modifiers are those adjuvants that change the wetting, spreading or sticking characteristics of the spray solution. This is primarily accomplished by a modification in th...

This study investigated the adjuvant potential of W/O/W multiple emulsions and microemulsions, comparing them with traditional aluminum hydroxide and oil-in-water emulsion adjuvants against bluetongue vaccine (BTV). Local inflammatory reactions were assessed in rabbits by measuring the temperature of the animals and the skin thickness at the site of application. Antibodies titers were determine...

How strong adjuvants such as complete Freund's adjuvant (CFA) promote T cell priming to protein antigens in vivo is still unclear. Since the unmethylated CpG motifs in DNA of bacteria and other nonvertebrates are stimulatory for B cells and antigen-presenting cells, the strong adjuvanticity of CFA could be attributed, at least in part, to the presence of dead bacteria, i.e., a source of stimula...

Adjuvants are combined with vaccine antigens to enhance and modify immune responses, and have historically been primarily crude, undefined entities. Introducing toll-like receptor (TLR) ligands has led to a new generation of adjuvants, with TLR4 ligands being the most extensively used in human vaccines. The TLR4 crystal structures demonstrate extensive contact with their ligands and provide clu...

Enteric redmouth disease (ERM) caused by the fish pathogen Yersinia ruckeri is a major threat to freshwater production of rainbow trout throughout all life stages. Injection vaccination of rainbow trout against Y. ruckeri infection has been shown to confer better protection compared to the traditionally applied immersion vaccination. It may be hypothesized, based on experience from other vaccin...