Previous studies showed that cultured mouse trophoblast stem cells (mTSCs) have the most rapid proliferation, normal maintenance of stemness/potency, the least spontaneous differentiation, and the lowest level of stress-activated protein kinase (SAPK) when incubated at 2% O2 rather than at the traditional 20% O2 or hypoxic (0.5% and 0% O2) conditions. Switching from 2% O2 induced fast SAPK resp...

The article is devoted to the analysis of flame anchoring mechanism in test case MASCOTTE C-60 RCM2 on supercritical hydrogen/oxygen combustion at 60 bar, with transcritical (liquid) injection oxygen. simulated by means in-house parallel code HeaRT three-dimensional LES framework. cubic Peng–Robinson equation state its improved translated volume formulation assumed. Diffusive mechanisms and tra...

[1] Wetland soil oxygen (O2) is rarely measured, which limits our understanding of a key regulator of nitrogen loss through denitrification. We asked: (1) How does soil [O2] vary in riparian wetlands? (2) How does this [O2] variation affect denitrification rates and end products? and (3) How does [O2] variation and previous exposure to O2 affect trace gas fluxes? We collected a continuous seven...

The long-term benefits of nitroglycerin (NTG) therapy are limited by the development of vascular tolerance and endothelial dysfunction in conductance coronary arteries. We have determined whether nitrate tolerance extends to NTG effects on myocardial O2 consumption (MV(O2)) and the ability of endogenous nitric oxide (NO) to modulate MV(O2) during exercise. In chronically instrumented dogs (n = ...

The aim of the present study was to determine the impact of oxygen concentration during in vitro culture of human oocytes and embryos on fertilization, cleavage, implantation, pregnancy, multiple gestation and abortion rates. Women 20-48 years old presenting for infertility treatment and accounting for 3484 in vitro fertilization/intracytoplasmic sperm injection cycles were included in the stud...

PURPOSE This study compared the effect of supplemental oxygen (O2) on pediatric patients' apnea status and oxyhemoglobin saturation during: 1) conscious sedation for dental procedures and 2) the recovery period following sedation. METHODS Fourteen child patients (mean age 42 months) sedated with 50 mg/Kg chloral hydrate, 25 mg hydroxyzine pamoate, and 1.5 mg/Kg meperidine were treated for two...

Energy metabolism is intrinsic to cell viability but surprisingly has been little studied in human embryonic stem cells (hESCs). The current study aims to investigate the effect of environmental O2 tension on carbohydrate utilisation of hESCs. Highly pluripotent hESCs cultured at 5% O2 consumed significantly more glucose, less pyruvate and produced more lactate compared to those maintained at 2...

Human embryonic stem cells (hESCs) have great potential for clinical therapeutic use. However, relatively little is known of the mechanisms which dictate their specificity of adhesion to substrates through adhesion proteins including integrins. Previous observations demonstrated enhanced clonogenicity in reduced oxygen culture systems. Here, we demonstrated via antibody blocking experiments tha...

The purpose of these studies was to test whether O2 tension influences cardiotoxicity due to doxorubicin. Isolated hearts were perfused by the method of Langendorff at constant pressure with Krebs-Henseleit buffer saturated with 5% CO2 and either 95% or 20% O2. Toxicity due to doxorubicin was evaluated from changes in heart rate and from uptake of trypan blue by nonviable nuclei. Heart rate was...

We have measured maximal oxygen consumption ([Formula: see text]O2,max) of mice lacking one or two of the established mouse red-cell CO2 channels AQP1, AQP9, and Rhag. We intended to study whether these proteins, by acting as channels for O2, determine O2 exchange in the lung and in the periphery. We found that [Formula: see text]O2,max as determined by the Helox technique is reduced by ~16%, w...