نتایج جستجو برای: nucleic acid amplification

تعداد نتایج: 804699  

Journal: :Clinica chimica acta; international journal of clinical chemistry 2006
Salvatore A E Marras Sanjay Tyagi Fred Russell Kramer

BACKGROUND A number of formats for nucleic acid hybridization have been developed to identify DNA and RNA sequences that are involved in cellular processes and that aid in the diagnosis of genetic and infectious diseases. METHODS The introduction of hybridization probes with interactive fluorophore pairs has enabled the development of homogeneous hybridization assays for the direct identifica...

2009
Johan Stenberg Jan Grawé Jenny Göransson Anna Karman Magnus Elgh Mathias Howell Mats Nilsson Jonas Jarvius

Using an amplified single molecule detection platform based on padlock probe and proximity ligation, detection of very low amounts of nucleic acid or protein molecules is achieved. Protein and nucleic acid analyses are performed in a similar fashion using circularizing DNA probes, rolling circle amplification and fluorescence-based counting of single reporter molecules. This allows a single sys...

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