Carrier proteins are 80- to 100-residue way stations that are central to polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) enzymatic assembly lines. Because the biosynthetic intermediates for catalytic operations are presented on carrier proteins as covalently attached thioesters (via a 4'-phosphopantetheine prosthetic group), the specific protein-protein interactions between...

Marine symbiotic actinomycetes play a key role in drug development and their ecological niches can influence variety of natural product biosynthesis, providing potential defensive benefits. In this study, we report the whole-genome sequence analysis marine gastropod mollusk Planaxis sp.-associated Streptomyces griseus SCSIO PteL053, which harbors 28 putative biosynthetic gene clusters (BGCs). A...

In this study, the function of a non-ribosomal peptide synthetase-like (NRPS-like) encoding gene AOL_s00188g306 ( g306 ) was investigated to reveal association between NRPS and nematocidal activity in nematode-trapping fungus Arthrobotrys oligospora . Sequence analysis indicated that encoded product is an adenylation domain synthetases extended short-chain dehydrogenase/reductase domain-contain...

The issues of farmers’ old-age security and land use have long been the focus Chinese scholars’ governmental attention. Land transfer plays a vital role in promoting agricultural scale operations, adjusting structures, improving utilization, while function is one important factors affecting transfer. Based on data China Health Retirement Longitudinal Study (CHARLS), this study uses probit struc...

Nonribosomal peptide synthetases (NRPSs) assemble structurally complex peptides from simple building blocks such as amino and carboxyl acids. Product release by macrocyclization or hydrolysis is catalyzed by a thioesterase domain that is an integrated part of the NRPS enzyme. A second thioesterase of type II (TEII) encoded by a distinct gene associated with the NRPS cluster was previously shown...

The epothilones are potent anticancer natural products produced by a polyketide synthase (PKS)-nonribosomal peptide synthetase (NRPS) hybrid involving proteins EpoA-F. The single NRPS module of the epothilone assembly line, EpoB, is a distinct subunit of approximately 160 kDa and consists of four successive domains: cyclization, adenylation, oxidation, and peptidyl carrier protein (Cy-A-Ox-PCP)...

Intensive study of gene diversity of bioactive compounds in a wood-rot fungus, Xylaria sp. BCC1067, has made it possible to identify polyketides and nonribosomal peptides (NRPs) unaccounted for by conventional chemical screening methods. Here we report the complete nonribosomal peptide synthetase (NRPS) gene responsible for the biosynthesis of an NRP, bassianolide, using a genetic approach. Iso...

Plant growth-promoting rhizobacteria (PGPR) are a group of rhizosphere bacteria that promote plant growth. Delftia tsuruhatensis MTQ3 is a member of PGPR that produces siderophores. The draft genome sequence of MTQ3 has been reported. Here, we analyzed the genome sequence of MTQ3 and performed a comparative genome analysis of four sequenced Delftia strains, revealing genetic relationships among...

Streptomyces clavuligerus, an industrially important producer of clavulanate as well as cephem antibiotics, also produces the N-acylated dithiolopyrrolone antibiotic holomycin, a reported inhibitor of RNA synthesis. The genome sequence of S. clavuligerus ATCC 27064 was examined for a potential biosynthetic gene cluster, assuming that holomycin arises from some derivative of an L-Cys-L-Cys dipep...

Harmony Search Algorithm (HSA) is a relatively new nature-inspired algorithm. It evolves solutions in the problem search space by mimicking the musical improvisation process in seeking agreeable harmony measured by aesthetic standards. The Nurse Rostering Problem (NRP) is a well-known NP-hard scheduling problem that aims at allocating the required workload to the available staff nurses at healt...