Abstract Given the availability of technological solutions and guidelines for safe drinking water, direct potable reuse reclaimed water has become a promising option to overcome severe lack in arid regions. However, growing awareness presence antibiotic-resistant bacteria (ARB) antibiotic resistance genes (ARG) corresponding raw wastes led new safety concerns. This study investigated fate ARB i...

In this study, an efficient Agrobacterium-mediated transformation method was developed forpomegranate (Punica granatum L.), a difficult-to-transform plant. In vitro shoot segments wereinoculated with Agrobacterium tumefaciens strain LBA4404 harboring the binary vectorpBI121 carrying the neomycin phosphotransferase (nptII) gene as a selectable marker and β-glucuronidase (gus) gene as a reporter....

BACKGROUND The future of genetic transformation as a tool for the improvement of fruit trees depends on the development of proper systems for the assessment of unintended effects in field-grown GM lines. In this study, we used eight transgenic lines of two different citrus types (sweet orange and citrange) transformed with the marker genes β-glucuronidase (uidA) and neomycin phosphotransferase ...

Abstract Background One of the parameters required for assessment food and feed safety is detection identification genetically modified organisms. Legislation in some countries necessitates quantification modification samples. Scientists have raised concern about antibiotic resistance marker (ARM) genes used transformation crops intended human animal consumption. In present work two molecular a...

Whitefly (Bemisia tabaci) is a polyphagous insect that causes huge damage in several horticultural crops, including tomato, by sucking nutrients from the phloem and transmitting viruses. Whiteflies are particularly difficult to manage use of chemicals remains common practice, which development insecticidal resistance. Thus, there considerable interest introduction resistance whitefly classical ...

BACKGROUND The ability to compose biological systems from smaller elements that act independently of the other upon assembly may help make the forward engineering of biological systems practical. Engineering biology in this manner is made difficult by the inherent nonlinear response of organisms to genetic devices. Devices are inevitably coupled to one another in the cell because they share the...

Luxembourg notified to the European Commission its scientific arguments justifying the implementation of a national safeguard measure prohibiting the placing on the market of GM potato EH92527-1 for cultivation purposes in Luxembourg, after which the European Commission asked the European Food Safety Authority (EFSA) to assess the scientific information supporting the prohibition. Having consid...

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Sugarcane (Saccharum officinarum L.) is the most important food and energy crop worldwide. In present study, an efficient Agrobacterium mediated transformation regeneration system for sugarcane cultivar SPF-234 was established. tumefaciens strains EHA101and LBA4404 using vector pIG121 Hm, having GUS, HPTII NPTII genes were used. Polymerase chain reaction (PCR) histochemical assays confirmed GUS...