A procedure is presented for proline quantification in serum filtrates and urine hydrolysates. Interference from primary amino acids is greatly reduced by nitrozation. After nitrozation and neutralization, protine is reacted with ninhydrin in a concentrated electrolyte solution. The resulting red proline-ninhydrin product is salted out, extracted into benzene, and measured at 520 nm. The extent...

The chemical composition of a fingermark potentially holds a wealth of information about the fingermark donor, which can be extracted by immunolabeling. Immunolabeling can be used to detect specific components in fingermarks; however, to be applicable in the forensic field, it should be compatible with commonly used fingerprint visualization techniques. In this study, the compatibility of immun...

STRONGLY ALKALINE SOLUTIONS ninhydrin (1,2,3 indantrione hy) combines with guanidine, monosubstituted guanidines, and disubstituted guanidines to form highly fluorescent addition ucts (1). This reaction has been used as the basis for a simple, itive fluorimetric method for the determination of creatine in m, blood, and urine. he most widely used methods for determining creatine are based n the ...

Ninhydrin was implemented as the primary police method of developing latent fingermarks on paper, cardboard and some other porous surfaces from late 1960s. Some researchers have used individual amino acids, or mixtures a testing effectiveness reagent formulations. It not however known whether simple acids could effectively emulate in reactions with reagents such ninhydrin. The first part this s...

The determination of the amino acid composition of peptides and proteins is usually carried out on acid hydrolysates by ion-exchange chromatography, by following the methods developed by Moore & Stein and their collaborators (Moore, Spackman & Stein, 1958). This method is precise but time-consuming, requiring up to 600 individual determinations for a single protein analysis. Automatic scanning ...

SERUM CREATINE KINASE activity has beell measured by determining the amount of creatine liberated in the following reaction: ADI + creatine phosphate ATP + creatine. The Voges-Proskauer reactioii, production of a color by reaction of creatille with diacetyl alid -naphthol, has heeii used to measure enzymatically released creatine (1-5). The presence of a sulfhydryl compound in tile incubatioii ...