Protein-protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellular activities, targeted-drug design, and whole cell engineering. A large-scale comprehensive pull-down assay was performed using a His-tagged Escherichia coli ORF clone library. Of 4339 bait proteins tested...

FhuA (Mr 78,992, 714 amino acids), siderophore receptor for ferrichrome-iron in the outer membrane of Escherichia coli, was affinity tagged, rapidly purified, and crystallized. To obtain FhuA in quantities sufficient for crystallization, a hexahistidine tag was genetically inserted into the fhuA gene after amino acid 405, which resides in a known surface-exposed loop. Recombinant FhuA405.H6 was...

Ku is an ubiquitous nuclear heterodimeric protein consisting of p70 and p86 subunits that binds double-stranded DNA termini and associates with chromosomes in vivo. It was originally described as an autoantigen in patients with certain autoimmune diseases. The individual subunits of Ku have been difficult to isolate from human cells without denaturation and attempts to produce functional recomb...

The alpha1- and beta1-subunits of human soluble guanylate cyclase (sGC) were coexpressed in the Sf9 cells/baculovirus system. In addition to the native enzyme, constructs with hexahistidine tag at the amino and carboxyl termini of each subunit were coexpressed. This permitted the rapid and efficient purification of active recombinant enzyme on a nickel-affinity column. The enzyme has one heme p...

Don Cupitt’s version of religious non-realism based as it is on linguistic constructivism, radical relativism and the view that culture forms human nature has been attacked with devastating effect by realists in the last few years. I argue that there is another strand in Cupitt’s thinking, his biological naturalism, that supports a different version of religious non-realism and that he failed t...

Abstract This study describes the concept of religious moderation according to Yusuf Al-Qardhawi, Quraish Shihab, and Salman al-Farisi. research is caused by current reality that considered a new understanding in Islam, even though has existed since time Prophet Muhammad SAW. So this first step examine moderation. The type used qualitative with method library (library research) through stages o...

in the abbasid era, chieftainship (niqābat) was a politico-religious institution with social functions. since the early periods of the samanids era, the alavids’ chieftainship in nayshābūr, capital of khurāsān, had been turned over to the ḥusaynī alavids of banū zubāra, but amīr nūḥ b. manṣūr sāmānī (ruled 366-378) handed over this post to a ḥasanī alavid, whose descendants became the hereditar...