The diagnosis of nongenital Neisseria gonorrhoeae infection by the Becton Dickinson ProbeTec ET Chlamydia trachomatis and N. gonorrhoeae Amplified DNA assay had low positive predictive value among an Australian community-based sample of homosexual men. Only 30.4% of oropharyngeal samples and 73.7% of anorectal samples were positive for N. gonorrhoeae by the porA assay. The accuracy of nucleic a...

Standard and experimental tests were used by a reference diagnostic laboratory to determine the identity of 182 "suspected" Neisseria gonorrhoeae isolates submitted by state health departments because of inconclusive laboratory results. More than 97% of these cultures were subsequently identified by a rapid microcarbohydrate test in conjunction with confirmatory immunological procedures. The ex...

Two conventional PCR-enzyme immunoassays (PCR-EIAs) and two real-time PCR assays (LightCycler system; Roche Diagnostics) were evaluated as confirmation assays with cppB and 16S rRNA genes as targets. Of 765 male and female genitourinary and nasopharyngeal specimens positive for Neisseria gonorrhoeae in the COBAS AMPLICOR Chlamydia trachomatis/Neisseria gonorrhoeae PCR test (Roche Diagnostics), ...

A selective identification medium for Neisseria gonorrhoeae was devised by incorporating antibiotics and a mixture of sugars into a yeast extract agar. The results were compared with those using a standard combined selective/non-selective blood agar medium. Recovery rates of N gonorrhoeae from specimens from 208 patients cultured on both media were comparable, but the selective descriptive medi...

Gonococcal urethritis is common with HIV, but gonococcal arthritis is rare. We report two HIV-positive patients with gonococcal arthritis and review previously published reports. A 27-year-old HIV-positive female presented with a pustular skin rash and acute oligoarthritis. Neisseria gonorrhoeae was cultured from the right elbow aspirate. The second patient, a 24-year-old HIV-positive female on...

Abstract Background The prevalence of azithromycin resistance in Neisseria gonorrhoeae is increasing numerous populations worldwide. Objectives To characterize the genetic pathways leading to high-level resistance. Methods A customized morbidostat was used subject two N. reference strains (WHO-F and WHO-X) dynamically sustained pressure. We tracked stepwise evolution by whole genome sequencing....

An evaluation of fluorescent amplified fragment length polymorphism (FAFLP) for typing Neisseria gonorrhoeae using 52 isolates revealed that its discriminatory power approached that of Opa-typing in identifying epidemiologically linked isolates. Automated, accurate sizing of FAFLP amplified fragments permits objective data analysis and storage, making it an attractive method for large surveilla...

The commercial BD ProbeTec ET (BDPT) system for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis from clinical specimens was compared with our in-house LightCycler real-time PCR (LC-PCR) assays. Specimens initially positive by the BDPT system were retested by our LC-PCR assays. Our results for C. trachomatis testing indicate a 91.2% agreement when the results of 114 clinical spe...

A new self-contained medium system for the cultivation of Neisseria gonorrhoeae was compared to modified Thayer-Martin medium for the diagnosis of gonorrhea from endocervical specimens. There was no difference in the ability of the two methods to support the growth of N. gonorrhoeae.

A penicillin-resistant Neisseria gonorrhoeae strain was isolated. The resistance was due to the production of TEM-1 beta-lactamase encoded by a plasmid. This 6.6-kilobase plasmid was compared with the previously known 7.4- and 5.3-kilobase penicillin R plasmids of N. gonorrhoeae.