A HPLC method for simultaneous determination of diclofenac sodium and papaverine hydrochloride in tablets was developed and validated. The determination was performed with a Zorbax SB-C18 column, mobile phase: methanol-water (60:40, v/v), flow rate: 1 mL min(-1) and UV detection at 278 nm.

A simple, sensitive, rapid, precise and economical reverse phase HPLC method has been developed for the estimation of valsartan from pharmaceutical dosage forms. The was carried out using Chromatographic conditions were established by employing a C18 analytical phenomenal (Kinetex) column (5micron -C18, 250x4.6mm) with mobile phosphate buffer (PH 3.5) acetonitrile in 60:40 ratio. sample injecte...

a validated hplc method was developed to determine doxorubicin concentration in a small volume of rat plasma (60 µl) with convenient fluorescence detection. sample preparation includes a simple one-step liquid-liquid extraction using minimum amount of organic solvent, with extraction recoveries more than 95%. the analysis was accomplished using perfectsil c18 column maintained at 35 °c and a mo...

In this article, the diocotron modes developing in a coaxial diode and resonant magnetron forming six-vane A6 relativistic both fed by split cathode are studied Particle-in-Cell simulations. The is novel type of which sustains column longitudinally oscillating electrons (smooth bore) or over non-emitting axial conductor. connection between longitudinal oscillatory dynamics mode development, as ...

This study involves the incorporation of a commercially available Phenomenex Onyx C18 monolith column into the separation and detection of oxidative DNA damage. It includes thorough investigation of monolith performance and a comparison of the performance of monolith columns with a commercially available packed Restek reverse phase Ultra C18 column for the separation of DNA bases and nucleoside...

A glutathione S-transferase (GST, EC 2.5.1.18) inhibitor was isolated from the onion bulb by fractionation and purification using Sep-pak Vac C18 column, TLC and HPLC. Five partially purified onion bulb GSTs (GSTa and GSTb as minor GSTs, and GSTc, GSTd and GSTe as dominant GSTs) separated by DEAE column were used to detect the inhibitory substance in onion bulb extract. Among the three fraction...

This application note describes a fast UHPLC method for the determination of 20 ginsenosides. The developed method allows for the determination of 20 ginsenosides in less than 35 minutes. A binary high pressure gradient is used at a flow rate of 0.6 ml/min. By applying a Bluespher 100-2 C18 column in the dimension 150 x 2 mm ID, the total run time could be reduced to less than 41 minutes when a...

Generating chemical libraries in droplet form is vital to the adoption of microdroplet systems in high-throughput screening (HTS). This paper presents microfractionation in droplets (μFD), a method for generating heterogeneous droplet libraries using standard separation processes. In μFD, compounds eluting from a separation column are permanently encapsulated into droplets by a droplet generato...