Material on the surface of activated T-cells was displaced following incubation with a sulfated polysaccharide, dextrin 2-sulfate (D2S), and purified by anion-exchange chromatography. This revealed a complex comprising histones H2A, H2B, H3, and H4 and DNA fragmented into 180-base pair units characteristic of mono-, di-, tri, and polynucleosomes, a pattern of fragmentation similar to that found...

Amberlite XAD-2 was used to extract bile acids from urine or diluted serum of patients with hepatobiliary diseases. Columns containing Sephadex LH-20 were then used to separate the sulfated and nonsulfated bile acids. Thin-layer chromatography of the sulfated bile acid fraction obtained from urine revealed several spots with R(F) values different from those of the taurine or glycine conjugates....

Genital human papillomaviruses (HPV) represent the most common sexually transmitted agents and are classified into low or high risk by their propensity to cause genital warts or cervical cancer, respectively. Topical microbicides against HPV may be a useful adjunct to the newly licensed HPV vaccine. A main objective in the development of novel microbicides is to block HPV entry into epithelial ...

The study of the relationship between the complex structures and numerous physiological functions of the glycosaminoglycans (GAGs) heparin and heparan sulfate (HS) has continued to thrive in the past decade. Though it is clear that the monosaccharide sequences of these polysaccharides must determine their ability to modulate the action of growth factors, morphogens, chemokines, cytokines, and m...

The sulfation of tyrosine residues is a posttranslational modification that occurs in all eukaryotes.[1] This ubiquitous modification has been identified on many secretory proteins with diverse biological activities. Tyrosine O-sulfate (Tyr(SO3H)) residues often are important for facilitating specific protein±protein interactions.[2] However, there is little information on the molecular basis f...

the polysaccharides of acanthophora spicifera and cystoseira trinode were isolated and theircomponents identified using different chemical and spectral techniques. their effects were evaluated for thefirst time on hyperlipidemic rats. atorvastatin ca (lipitor®) was used as a reference drug. results revealedthat the polysaccharides isolated were of sulfate type. acanthophora spicifera lowered th...

A high molecular weight chondroitin sulfate proteoglycan (M, 240,000) is released from platelet surface during aggregation induced by several pharmacological agents. Some details on the structure of this compound are reported. &Elimination with alkali and borohydride produces chondroitin sulfate chains with a molecular weight of 40,000. The combined results indicate a proteoglycan molecule cont...

Three different fucoidan fractions were isolated and purified from the brown alga, Sargassum mcclurei. The SmF1 and SmF2 fucoidans are sulfated heteropolysaccharides that contain fucose, galactose, mannose, xylose and glucose. The SmF3 fucoidan is highly sulfated (35%) galactofucan, and the main chain of the polysaccharide contains a →3)-α-L-Fucp(2,4SO₃⁻)-(1→3)-α-L-Fucp(2,4SO₃⁻)-(1→ motif with ...

Because of the frequent association of metachromatic staining material and lipid deposits within the artery wall, the suggestion has been made that the mucopolysaccharides of the connective tissue of arteries may in some way render lipids of the blood insoluble and thereby set the stage for the development of the lesions of arteriosclerosis." "' Studies in this laboratory have demonstrated the ...

Decorin and its associated glycosaminoglycan (GAG) side chain, dermatan sulfate (DS), play diverse roles in soft tissue formation and potentially aid in the mechanical integrity of the tissue. Deeper understanding of the distribution and orientation of the GAGs on a microscopic level may help elucidate the structure/function relationship of these important molecules. The hypothesis of the prese...