Na,K-ATPase is a hetero-oligomer of an aand a b-subunit. The a-subunit (Na,K-a) possesses the catalytic function, whereas the bsubunit (Na,K-b) has cell-cell adhesion function and is localized to the apical junctional complex in polarized epithelial cells. Earlier, we identified two distinct conserved motifs on the Na,K-b1 transmembrane domain that mediate protein-protein interactions: a glycin...

Gomes, Pedro, and Patrı́cio Soares-da-Silva. Upregulation of apical NHE3 in renal OK cells overexpressing the rodent 1-subunit of the Na pump. Am J Physiol Regul Integr Comp Physiol 290: R1142–R1150, 2006. First published November 17, 2005; doi:10.1152/ajpregu.00102.2005.— Vectorial Na reabsorption across the proximal tubule is mediated by apical entry of Na , primarily via Na /H exchanger isofo...

Ouabain inhibited 86RbCl uptake by 80% in rabbit gastric superficial epithelial cells (SEC), revealing the presence of a functional Na+,K+-ATPase [(Na+ + K+)-transporting ATPase] pump. Intact SEC were used to study the ouabain-sensitive Na+,K+-ATPase and K+-pNPPase (K+-stimulated p-nitrophenyl phosphatase) activities before and after lysis. Intact SEC showed no Na+,K+-ATPase and insignificant M...

Na/K pump current was determined between -140 and +60 mV as steady-state, strophanthidin-sensitive, whole-cell current in guinea pig ventricular myocytes, voltage-clamped and internally dialyzed via wide-tipped pipettes. Solutions were designed to minimize all other components of membrane current. A device for exchanging the solution inside the pipette permitted investigation of Na/K pump curre...

Introduction: To assess the salivary composition of proteins and minerals in smokers compared with non-smokers.   Materials and Methods: In this study we compared the total protein and Ca, Na, K, Mg, Pb of whole saliva in two groups of men (28 smokers and 31nonsmokers) aged between 29-41years.   Results: Fifty-nine participants were evaluated. The mean age was 33.14±5.32 years among smo...

1. Measurements of the blood Na concentration and transepithelial electrical potential (T.E.P.) across Uca pugilator acclimated to sea water indicate that Na is maintained out of electrochemical equilibrium with sea water. The resulting net Na influx as well as the sodium gain due to ingestion of the medium must be balanced by extrarenal Na extrusion. 2. The small T.E.P. (-0.7 mV) and the 'tran...

a factorial experiment based on completely randomized design (crd) was performed with three replications to investigate the interaction between salinity and nh+4 / no-3 ratio on olive saplings. four levels of salinity (0, 50, 100,150 mm) and different ratios of nh+4/ no-3 (0:14, 2:12, 4:10, 6:8) were applied on one-year old olive saplings grown in perlite: sand media (1:1). results indicated th...