The human immunodeficiency virus type 2 (HIV-2) strain ROD/B can efficiently use the 7tm chemokine receptor CXCR-4 as a primary receptor to enter CD4-negative cells. We have stably expressed CXCR-4 on mink lung Mv-1-lu and feline kidney CCC cells (normally restrictive to HIV entry) and have shown efficient fusion, entry, and replication of ROD/B. Mutation of the two N-linked glycosylation sites...

Morganella morganii produces an inducible, chromosomally encoded AmpC beta-lactamase. We describe in this study three new variants of AmpC within this species with apparent pIs of 6.6 (M19 from M. morganii strain PP19), 7.4 (M29 from M. morganii strain PP29), and 7.8 (M37 from M. morganii strain PP37). After gene sequencing, deduced amino acid sequences displayed one to six substitutions when c...

Background While well known for its Viking past, Norway's population history and the influences that have shaped genetic diversity are less understood. This is particularly true with respect to demography, migration patterns, dialectal regions, despite there being curated historical records past several centuries. In this study, we undertook an analysis of mitochondrial DNA (mtDNA) within count...

Tth111II is a thermostable Type IIGS restriction enzyme that recognizes DNA sites CAARCA (R = A or G) and cleaves downstream at N11/N9. Here, the tth111IIRM gene was cloned and expressed in E. coli, and Tth111II was purified. The purified enzyme contains internally-bound S-adenosylmethionine (SAM). When the internal SAM was removed, the endonuclease activity was stimulated by adding SAM or its ...

Human solid tumors frequently have a relatively small growth fraction,which interferes with the action of many chemotherapeutic agents that target actively cycling cells. Several polyamine analogues are currently being developed for clinical application against human solid tumors including N1,N11-bis(ethyl)norspermine. Therefore, an effort was made to examine the effects of growth rate on polya...

The effects of active and passive finger movements on somatosensory potentials evoked by stimulation of the median nerve at the wrist or of finger I were investigated in 15 healthy volunteers. As compared to the resting condition, both active and passive movements of the stimulated hand fingers induced a marked reduction in the amplitude of the primary cerebral response (N20-P25 complex) as wel...

The theoretical approach utilizing bimodal Floquet theory in the quadrupolar/central-transition interaction frame, presented in an earlier article @J. D. Walls, K. H. Lim, and A. Pines, J. Chem. Phys. 116, 79 ~2002!#, is extended to describe the more complicated spin dynamics of I55/2 spin systems. Rotary resonance effects occur when the strength of the radio-frequency irradiation, v1 , matches...

N1,N11-diethylnorspermine (DENSPM) is a polyamine analog that down-regulates polyamine biosynthesis and potently upregulates the polyamine catabolic enzyme spermidine/spermine N1-acetyltransferase (SSAT). In certain cells, such as SKMEL-28 human melanoma cells, induction of SSAT is associated with rapid apoptosis. In this study, we used small interfering RNA (siRNA) to examine the role of SSAT ...

We obtain a nontrivial upper bound for almost all elements of the sequences of real numbers which are multiplicative and at the prime indices are distributed according to the Sato–Tate density. Examples of such sequences come from coefficients of several L-functions of elliptic curves and modular forms. In particular, we show that |τ(n)| ≤ n11/2(log n)−1/2+o(1) for a set of n of asymptotic dens...

Nickel is an essential transition metal for the survival of Helicobacter pylori in the acidic human stomach. The nickel-responsive transcriptional regulator HpNikR is important for maintaining healthy cytosolic nickel concentrations through the regulation of multiple genes, but its complete regulon and role in nickel homeostasis are not well understood. To investigate potential gene targets of ...