The NEUROGENINS (NGNs) are neural-specific basic helix-loop-helix (bHLH) transcription factors. Mouse embryos lacking ngn1 fail to generate the proximal subset of cranial sensory neurons. ngn1 is required for the activation of a cascade of downstream bHLH factors, including NeuroD, MATH3, and NSCL1. ngn1 is expressed by placodal ectodermal cells and acts prior to neuroblast delamination. Moreov...

The linked Mrf4 and Myf5 genes encode two transcription factors essential for the determination and differentiation of skeletal muscle in the embryo. The locus is controlled by a multitude of interdigitated enhancers that activate gene expression at different times and in precisely defined progenitor cell populations. Manipulation of the enhancer-promoter composition of the locus reveals a nove...

Hormone replacement therapy (HRT) is used in postmenopausal women to relieve symptoms of menopause and prevent osteoporosis. We sought to evaluate changes in mRNA expression of key myogenic factors in postmenopausal women taking and not taking HRT following a high-intensity eccentric resistance exercise. Fourteen postmenopausal women were studied and included 6 control women not using HRT (59 +...

Introduction: The objective of the present research was to determine the effect of low-intensity eccentric training with blood flow restriction (BFR) on Hepatocyte Growth Factor (HGF) and myogenic factor 5 (Myf5), in non-athletes young men. Methods: In this study, 20 non-athlete young men were allocated to two eccentric training and eccentric training with BFR groups. Eccentric training progra...

All skeletal muscle progenitor cells in the body derive from the dermomyotome, the dorsal epithelial domain of developing somites. These multipotent stem cells express Pax3, and this expression is maintained in the myogenic lineage where Pax3 plays an important role. Identification of Pax3 targets is therefore important for understanding the mechanisms that underlie the onset of myogenesis. In ...

Asymmetric cell division is a conserved mechanism to generate progeny with divergent fates. In the context of somatic stem cells, it provides a mode of selfrenewal that retains the stem cell identity of one daughter cell while producing another daughter cell that is committed to differentiation. This allows for a balance between the requirement for committed progenitors during regeneration and ...

We describe the effects of an absence of retinoic acid (RA) on the development of somites in the quail embryo. RA was removed by generating vitamin A deficient quail embryos whereupon the resulting defects in the embryos can be analysed. The effect on the somites is threefold. Firstly, they are half the size of normal, but the total number of somites is the same as normal. There has therefore b...

It is predicted that already in 2040, 35% of requirements for meat will be provided by vitro production. Recreating the course myogenesis vitro, and thus resembling a structure muscle tissue, basis research focusing on obtaining cultured requires providing relevant factors supporting proliferation satellite cells—being precursors skeletal muscles. The present work aimed to develop composition m...

The tongue is a muscular organ and plays a crucial role in speech, deglutition and taste. Despite the important physiological functions of the tongue, little is known about the regulatory mechanisms of tongue muscle development. TGFβ family members play important roles in regulating myogenesis, but the functional significance of Smad-dependent TGFβ signaling in regulating tongue skeletal muscle...