One of the main reasons considered for BCG failure in tuberculosis-endemic areas is impediment by environmental mycobacteria in its processing and generation of memory T-cell response. To overcome this problem, we developed a unique lipopeptide (L91) by linking the promiscuous peptide (sequence 91-110) of 16 kDa antigen of Mycobacterium tuberculosis to Pam2Cys. L91 does not require extensive an...

Several antigens of Mycobacterium tuberculosis have been identified by monoclonal antibodies and are being exploited in the development of improved vaccines and diagnostic reagents, but none has been linked to a specific function. Herein we report that the 38-kDa extracellular lipoprotein antigen, the most potent immunogen of the mycobacteria, is a phosphate-binding protein with features very s...

Abstract Tuberculosis (TB), as a common infectious disease, still remains severe challenge to public health. Due the unsatisfied clinical needs of currently available diagnostic vehicles, it is desired establish new approach for universally detecting Mycobacterium tuberculosis. Herein, we designed real-time recombinase polymerase amplification (RPA) technology identifying M. tuberculosis within...

The local immune response to mycobacteria is complex, but mycobacterial antigen presentation by phagocytes to T helper cells is the pivotal interaction. Bacille Calmette-Guérin (BCG) vaccination is associated with the development of antituberculosis immunity but not necessarily with antitumor immunity. Animal studies have shown that an intact host immune system is required for the antitumor act...

Investigation of the immunologically active substance in Mycobacterium Zeprae has been hampered because of the unavail ability of pure cultures. However, this difficulty can now be circumvented in part by taking advantage of the identification of the group-specific antigen, Polysaccharide I (PolyINb ), present in Nocardia and Mycobacteria ( I. 3 G. 8) . This has been chemically characterized as...

Despite decontamination, overgrowth by pseudomonads renders cultural isolation of mycobacteria from respiratory specimens of patients with cystic fibrosis (CF) difficult or impossible. We performed a prospective study by comparing levels of reduction of overgrowth and recovery of mycobacteria using either pretreatment with N-acetyl-L-cysteine (NALC)-NaOH alone or pretreatment with NALC-NaOH and...

non-tuberculosis mycobacteria (ntm), as certain species of mycobacteria, can affect human in several ways. in the preceding years, the rate of ntm detection has risen in both immunocompromised and immunocompetent patients. on the other hand, several reports have debated the possibility of co-infection of both mycobcateriu tuberculosis (mtb) and ntm in individuals that puts the role of ntm in di...

Background: Technicians working in high burden tuberculosis (TB) laboratories pose a higher risk of being infected by Mycobacterium from clinical samples. Contamination control is mandatory to detect the release bacteria into environment and minimize exposure workers. The contamination measurement rarely performed due lack standard methodology. This study optimized applied unique culture-based ...

Studies of Mycobacterium leprae, Mycobacterium tuberculosis and Nocardia caviae in comparison with each other and with other Mycobacterium and Nocardia species were performed on the basis of antigen 21 intramolecular heterogeneity. Three different antisera were used: rabbit anti-Mycobacterium smegmatis antiserum, rabbit anti-Nocardia asteroides antiserum, and a lepromatous serum pool. With refe...