نتایج جستجو برای: multiplex polymerase chain reaction

تعداد نتایج: 697832  

Journal: :Environmental Health Perspectives 1996
K S Brown

The sequential deletion method is generally used to locate the functional domain of a protein. With this method, in order to find the various N-terminal truncated mutants, researchers have to investigate the ATG-like codons, to design various multiplex polymerase chain reaction (PCR) forward primers and to do several PCR experiments. This web server (N-terminal Truncated Mutants Generator for c...

Journal: :Revista da Sociedade Brasileira de Medicina Tropical 2016
Abazar Pournajaf Ramazan Rajabnia Mansour Sedighi Aziz Kassani Vahid Moqarabzadeh Lida Lotfollahi Abdollah Ardebilli Behzad Emadi Gholamreza Irajian

INTRODUCTION: This study aimed to determine the prevalence, and virulence factors of Listeria monocytogenes isolated from various samples by multiplex polymerase chain reaction (MPCR). METHODS: A total of 617 isolates were obtained and MPCR was employed for detection of the inlA, inlC, and inlJ genes. RESULTS: L. monocytogenes was detected in 46 (7.45%) of the 617 specimens. inlA, inlC, a...

Journal: :Applied and environmental microbiology 1991
A K Bej S C McCarty R M Atlas

Multiplex polymerase chain reaction (PCR) and gene probe detection of target lacZ and uidA genes were used to detect total coliform bacteria and Escherichia coli, respectively, for determining water quality. In tests of environmental water samples, the lacZ PCR method gave results statistically equivalent to those of the plate count and defined substrate methods accepted by the U.S. Environment...

Journal: :Twin research and human genetics : the official journal of the International Society for Twin Studies 2006
Wenjing Gao Liming Li Weihua Cao Siyan Zhan Jun Lv Ying Qin Zengchang Pang Shaojie Wang Weijian Chen Rongfu Chen Yonghua Hu

This study reports on the determination of zygosity in Chinese adult twins by simple questionnaire and physical features comparison. The subjects were 511 twin pairs from two cities and their town areas, consisting of 371 monozygotic (MZ) and 140 same-sex dizygotic (DZ) pairs, identified by ABO blood group and multiplex polymerase chain reaction of several polymorphic short tandem repeat marker...

2007
Emma J. Parkin Thirsa Kraayenbrink Jean Robert M.L. Opgenort George L. van Driem Nirmal Man Tuladhar Peter de Knijff Mark A. Jobling

Twenty-six Y-chromosomal short tandem repeat (STR) loci were amplified in a sample of 769 unrelated males from Nepal, using two multiplex polymerase chain reaction (PCR) assays. The 26 loci gave a discriminating power of 0.997, with 59% unique haplotypes, and the highest frequency haplotype occurring 12 times. We identified novel alleles at four loci, microvariants at a further two, and nine ex...

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