This study aimed to compare the sensitivity of multiplex PCR to ELISA technique in the instantaneous detection of HBV, HCV and HIVin blood samples from donors of the National blood Transfusion Centre in Togo. A total of 440 blood samplesfrom volunteer were collected and tested by ELISA and multiplex PCR for HBV, HCV and HIV detection. Among the 440 volunteer blood donors, 83% were female and 17...

Salmonellosis as an important zoonotic disease that causes food born poisoning in human through animalproducts and is considered as a worldwide public health hazard. Widespread studies have been conducted ondifferent aspects of incidence, treatment and control of salmonelosis all over the world. The aim of this studywas to evaluate the occurrence of widespread Salmonella serovars, S. typhimuriu...

enterococci are important because of their role as the leading cause of nosocomial infections which have a significant role in the dissemination and persistence of antimicrobial resistance genes. methods: in this study, we determined the distribution of enterococcal species in the sewage treatment plants in iran. furthermore, we improved a rapid and specific pcr method using primers (soda and d...

BACKGROUND & OBJECTIVES The conventional techniques used in TB diagnosis like AFB (acid fast bacilli) smear microscopy lack sensitivity and the gold standard, culture test takes time. A test based on multiplex polymerase chain reaction (PCR) targeting the 38 kDa gene and IS6110 insertion sequence, specific to Mycobacterium tuberculosis was developed to further increase the sensitivity of a TB-P...

Circulating tumor DNA (ctDNA) is emerging as a biomarker to detect molecular evidence of residual disease and predict recurrence in patients with colorectal cancer the adjuvant setting. Treatment paradigms have been evolving stage II III rectal total neoadjuvant therapy (TNT). Prospective evaluation, feasibility utility using ctDNA during clinical practice has limited date. In this retrospectiv...

INTRODUCTION Staphylococcus aureus, coagulase-negative staphylococci, Enterococcus spp., Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumanii have been found to be the most prevalent bacteremia-causing bacteria in patients with septicemia. Early detection of bloodstream infection (BSI) is crucial in the clinical setting. A multiplex PCR method for identification of these agent...

PURPOSE To develop a multiplex polymerase chain reaction (PCR) for the detection of adenovirus, herpes simplex virus, and Chlamydia trachomatis in conjunctival swabs. METHODS Oligonucleotide primers for detection of the 3 agents were combined in one reaction and evaluated for optimal performance using control DNAs of adenovirus type 2, herpes simplex virus, and C. trachomatis plasmid. The mul...

BACKGROUND & OBJECTIVES Bacillus anthracis, Yersinia pestis, Burkholderia pseudomallei and Brucella species are potential biowarfare agents. Classical bacteriological methods for their identification are cumbersome, time consuming and of potential risk to the handler. METHODS We describe a sensitive and specific multiplex polymerase chain reaction (mPCR) assay involving novel primers sets for...

BACKGROUND Multiplex polymerase chain reaction (mPCR) enables recovery of viruses from airways of patients with community-acquired pneumonia (CAP), although their clinical impact remains uncertain. METHODS Among consecutive adult patients who had undergone a mPCR within 72 hours following their admission to one intensive care unit (ICU), we retrospectively included those with a final diagnosi...

background: identifying regional types and evaluating the frequency of pneumococcal strains has become increasingly important especially in vaccination. the purpose of this study was the identification and frequency determination of our regional serotype and evaluation of the performance of recent type specific multiplex pcr for the diagnosis of streptococcus pneumonia serotypes. methods: all i...