The major pathway of mRNA degradation in yeast occurs through deadenylation, decapping and subsequent 5' to 3' exonucleolytic decay of the transcript body. To identify proteins that control the activity of the decapping enzyme, which is encoded by the DCP1 gene, we isolated a high-copy suppressor of the temperature-sensitive dcp1-2 allele, termed DCP2. Overexpression of Dcp2p partially suppress...

In vitro-transcribed (IVT) mRNA has come into focus in recent years as a potential therapeutic approach for the treatment of genetic diseases. The nebulized formulations IVT-mRNA-encoding alpha-1-antitrypsin (A1AT-mRNA) would be highly acceptable and tolerable remedy protein replacement therapy deficiency future. Here we show that lipoplexes containing A1AT-mRNA prepared optimum conditions coul...

هدف الباحث في البحث الحالي إلى التعرف علي دلالة الفروق الاندماج الجامعي لدى طلاب كلية التربية جامعة حلوان وفق متغيرات النوع الاجتماعي (ذكور، إناث) و الفرقة الدراسية (الأولي، الرابعة) ومحل الإقامة (ريف، حضر)، ولتحقيق أهداف الدراسة قام ببناء مقياس وتطبيقه عينة مكونة من (461) طالباً وطالبة، تراوحت أعمارهم الزمنية ما بين (17-23) سنة، بمتوسط عمري

background: although aberrant protamine (prm) ratios have been observed in infertile men, the mechanisms that implicit the uncoupling of prm1 and prm2 expression remain unclear. to uncover these mechanisms, in this observational study we have compared the prm1/prm2 mrna ratio and mrna contents of two regulatory factors of these genes. materials and methods: in this experimental study, sampling ...

The mRNA expression of growth hormone (GH) gene in early development stages of Siberian sturgeon was investigated using RT-PCR method. Samples were collected from unfertilized eggs up to 50 days post hatched (dph) larvae in 11 different times. Ribosomal protein L6 (RPL6) transcripts were used as the internal standard during quantification of GH mRNA expression. The results showed that the GH mR...

conclusions the obtained results indicate that rsm is an effective method for the optimization of culture conditions for hes gene expression in e. coli. results a maximum rhes mrna level of 376.16% was obtained under the following conditions: 0.6 mm iptg, 7 hours post-induction time, and 0.9 cell density before induction. the level of rhes mrna was significantly correlated with post-induction t...