Although tumor lysate contains all the potential helper and killer epitopes capable of stimulating T cells, it is difficult to use as a cancer vaccine because it suppresses dendritic cell (DC) function. We report that wild-type baculovirus possesses an adjuvant effect to improve the immunogenicity of tumor lysate. When mice were administered CT26 tumor cell lysate combined with baculovirus intr...

The purpose of this study is to establish a gene delivery system for interstitial tissue-specific protein expression in mice testes using modified recombinant baculovirus. Green fluorescent protein (GFP)-expressing recombinant baculovirus (GFP-baculovirus), in which the insect cell-specific polyhedron promoter was replaced by the cytomegalovirus (CMV)-IE promoter, was used to transfect testicul...

The application of molecular technologies, such as the expression of viral proteins in baculovirus, has provided a powerful approach to the diagnosis of human calicivirus (HuCV) infections. The baculovirus-expressed HuCV capsid protein self-assembles into virus-like particles, providing excellent reagents for immunologic assays, such as enzyme immunoassays (EIAs). Following the expression of th...

Methods Serum allergen-specific IgE of 105 subjects were quantified using ImmunoCAP and ImmunoCAP ISAC biochips. The subjects were classified based on a convincing clinical history and food challenge testing (FC) (dose=70g) to Penaeus monodon and Litopenaeus vannamei. Group1A: Either SA with FC positive to either shrimp (n =22) or SA admitted to emergency departments for severe reactions but no...

Influenza vaccines have long been manufactured in embryonated chicken eggs. This method has some problems such as a production period (about 6 months) and use of large amounts infectious pathogens. Recently, the recombinant subunit using baculovirus–insect cell system extensively investigated. In this system, viral immunodominant components can be produced more rapidly larger scale than convent...

The two economically important shrimp species in Bangladesh are the tiger shrimp, Penaeus monodon, and brown Metapenaeus monoceros. However, a continuous decline landing of these from industrial trawling made it critical to assess their stock biomass status explore response present degree removal. Given minimum data requirement robustness, this study employed depletion-based reduction analysis ...

In this review, we provide an overview of the current status of recombinant baculoviruses, describe the development of genetically engineered baculoviruses for use as rapid-action biological insecticides, and provide more detailed information on one particular set of recombinant viruses. The advantages and disadvantages of recombinant baculovirus insecticides, and the importance of risk-assessm...

background: vp4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. vp4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. methods: simian rotavirus sa11 gene 4 cdna was cloned into a cloning plasmid pdonrtm by recombination reaction using clonase ii enzyme mix. the resulting clone was c...

Two epizootics of cultured shrimp occurred in India in 1994. The first case of mass mortalities of tiger shrimp Penaeus monodon in July 1994 was very similar to that caused by yellowhead virus (YHV) infection with regard to gross clinical signs, host species and size of shrimp affected, but it was histologically atypical. Very interestingly, intranuclear inclusions typical of a white spot syndr...

Viral pathogens appear to exert the most significant constraints on the growth and survival of crustaceans under culture conditions. The prevalence of viral pathogens White Spot Syndrome Virus (WSSV), Hepatopancreatic Parvo Virus (HPV), Monodon Baculo Virus (MBV) and Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) in Penaeus monodon post-larvae was studied. Samples collected from ...