Nine selected hybridoma cell lines that produced monoclonal antibodies against the heat-labile enterotoxin encoded by a plasmid from an Escherichia coli strain of human origin (LTh) were characterized. Hybridomas that produced anti-LTh antibodies with previously unrecognized specificities or reactivities were selected for cloning. Each monoclonal antibody was tested for isotype and for binding ...

Class II HLA antigen expression in breast carcinoma and normal breast gland cells was compared using a method more accurate than immunofluorescence. This new method involves labeling membrane proteins with 131I and the anti-Class II HLA monoclonal antibody with 125I. The isolation and purification of the doubly labeled (125I-131I) immune complex was performed by affinity chromatography and chro...

Rimmelzwaan, G.F., van Es, J.H., Drost, G., UytdeHaag, F.G.C.M. and Osterhaus, A.D.M.E., 1991. Induction and characterization of monoclonal anti-idiotypic antibodies reactive with idiotopes of canine parvovirus neutralizing monoclonal antibodies. Vet. Immunol. Immunopathol., 29:139150. Monoclonal anti-idiotypic (anti-ld) antibodies (Ab2) were generated against idiotypes (ld) of canine parvoviru...

OBJECTIVE This study was designed to investigate the roles of programmed death-1 (PD-1) and PD-1 ligands (PD-L) in the development of murine acute myocarditis caused by Coxsackievirus B3. PD-1/PD-L belong to the CD28/B7 superfamily, and the PD-1/PD-L pathway is known to transduce a negative immunoregulatory signal that antagonizes the T-cell receptor-CD28 signal and inhibits T-cell activation. ...

The Thomsen-Friedenreich antigen (T-antigen) is a cryptic disaccharide structure on human erythrocytes and is supposed to be expressed in an unhidden form on carcinoma cells. We tested the ability of four antiT reagents (i.e., peanut agglutinili, human and rabbit anti-T antisera, and monoclonal anti-T antibodies) to agglutinate neuraminidase treated hu man erythrocytes and compared their capaci...

The immunogenicity in patients with melanoma, in monkeys, and in rabbits of four human melanoma-associated antigens (MA A) defined by murine monoclonal antibodies was investigated. The latter included the high molecular weight MAA and the (M, 115,000,100,000, and 95,000150,000 MAA. To this end sera from patients with melanoma, from monkeys, and from rabbits immunized with cultured human melanom...

Immunoassays are widely used for determination of the concentration of steroid hormones. However, obtaining specific anti-steroid monoclonal antibodies remains difficult. We used antibody engineering and phage display methods to change the specificity of an anti-cortisol monoclonal antibody towards estradiol. This work demonstrates that production of recombinant antibodies may be a valuable way...

Glycophorins are an important group of red blood cell (RBC) transmembrane proteins. Monoclonal antibodies against GPA employed in immunohistochemical staining during post-mortem examination: Through this method, it is possible to point out the RBC presence tissues. This experimental study aims investigate anti-GPA order evaluate vitality lesion from corpses different decomposition state. Six ca...

oral lichen planus is a chronic inflammatory immune-mediated disease. icam-1 and vcam-1 are vascular adhesion molecules that their receptors are located on endothelial cells and leukocytes. the aim of this study is the immunohistochemical evaluation of vcam1 and icam1 in oral lichen planus and to compare these two markers with normal mucosa for evaluation of angiogenesis. this descriptive-analy...