Thyroid cancer is the commonest classical endocrine tumor, accounting for approximately 1% of all cancers. The diagnosis of thyroid cancer is typically made on cytopathologic features on fine needle aspiration or histological features on surgical samples. Following treatment of patients with thyroidectomy, and in some cases radioiodine, patients are monitored for disease recurrence using a vari...

The use of molecular diagnostic techniques for bioprospecting and microbial diversity study purposes has gained more attention thanks to their functionality, low cost and quick results. In this context, ten degenerate primers were designed for the amplification of polyhydroxyalkanoate synthase (phaC) gene, which is involved in the production of polyhydroxyalkanoate (PHA)-a biodegradable, renewa...

Molecular pathology has become an essential adjunct in the realm of diagnostic histopathology. While gains in this field have been enjoyed in many areas, their impact has arguably been greatest felt in bone and soft tissue pathology. The purpose of this paper is to highlight the emerging role of molecular techniques in the context of paediatric bone pathology, discussing select syndromic and ne...

BACKGROUND New technologies for molecular analysis are increasing our ability to diagnose cancer. METHODS Several molecular analysis technologies are reviewed and their use in the clinical laboratory is discussed. RESULTS Select key technologies, including polymerase chain reaction and next-generation sequencing, are helping transform our ability to analyze cancer specimens. As these techno...

PURPOSE We diagnosed the subtypes of renal cell carcinoma on needle core biopsies using a combination of histopathology and a molecular diagnostic algorithm. MATERIALS AND METHODS Core biopsies were taken of renal tumors following nephrectomy. RNA was extracted and quantitative real-time polymerase chain reaction was performed for 4 gene products to differentiate among renal cell carcinoma su...

a School of Medicine, Genetic Diagnostic Laboratory Haig KaLaboratory Faye A. Eggerding, M.D., Director Georgetown University Medical Center, Molecular Diagnostic Laboratory Lee-Jun C. Wong, Ph.D., Director Mayo Clinic, Molecular Genetics Laboratory Stephen N. Thibodeau, Ph.D., Director Memorial Sloan-Kettering Cancer Center, Diagnostic Molecular Genetics Nathan A. Ellis, Ph.D., Director Myriad...

PURPOSE To optimize and streamline molecular genetics techniques in diagnosing choroideremia (CHM). METHODS PCR primers were designed for exons 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, and 15 of the CHM gene. Each PCR protocol was optimized so that all exons could be amplified with the same component ratio and PCR conditions. Sense and antisense primers were tested for their ability to be used as seq...