background: dendritic cells (dc) induce tumor or pathogen-specific t cell responses in humans. several laboratories have developed culture systems, including maturation factors for human dc from peripheral blood monocytes. we comprehensively compared standard maturation stimulus, an autologous monocyte-conditioned medium (mcm), with heparin for their ability to promote uniformly mature dc that ...

BACKGROUND The redox-active isoflavene anti-cancer drug, phenoxodiol, has previously been shown to inhibit plasma membrane electron transport and cell proliferation and promote apoptosis in a range of cancer cell lines and in anti-CD3/anti-CD28-activated murine splenocytes but not in non-transformed WI-38 cells and human umbilical vein endothelial cells. DESIGN AND METHODS We determined the e...

Recent studies have established the immunologic significance of the mixed lymphocyte interaction ( M L I ) l t h e proliferative reaction which occurs when lymphocytes from genetically disparate donors are mixed and cultured together. Even though the act ivi ty of cells in this system is assessed in terms of proliferation rather than the elaboration of a specific immunologic product, it is appa...

The lymphocyte proliferation in repeatedly studied mixed leukocyte cultures of peripheral white blood cells from a skin graft donor and 2 recipients was significantly increased at the time of graft rejection. This was determined from the increased proportions of mononuclear cells labeling with tritiated thymidine, increased mitotic indices, and the appearance of increased numbers of transformed...

hla-g is normally expressed on human trophoblast cells. it is a non-classical mhc molecule class i b. the role of hla-g in diabetic type 1 is not known. we investigated the role of ifn-β in induction hla-g expression on the monocyte derived dendritic cells (dc) in diabetes type 1. treatment of dendritic cell with ifn-β in vitro from diabetic patients (n=20) and normal subjects (n=20) resulted t...

Mycestericins A, B, C, D and E were isolated from the culture broth of Mycelia sterilia ATCC 20349 along with thermozymocidin (= myriocin). Their structures were elucidated on the basis of spectroscopic studies and chemical evidence. The acetate of mycestericin C was identical with the acetate of 6,7-dihydromyriocin. Mycestericins suppressed the proliferation of lymphocytes in the mouse allogen...

Splenic lymphocytes from preleukemic AKR mice are capable of participating in various cell-mediated immune responses. Spleen cells from AKR mice aged 1 to 10 months produced a significant graft-versus-host reaction when injected into 8-day-old AKR x C57Bl/6 F(1) hybrids. Splenic lymphocytes from similarly aged mice were also capable of responding to allogenic stimulation in a mixed lymphocyte r...

A new rapamycin producing culture was isolated and designated as Actinoplanes sp. N902-109. The addition of a cytochrome P-450 inhibitor and precursor feeding using this culture, and biotransformation approaches generated new rapamycin analogs with modifications at C-4, C-9, C-16, C-27, C-28 and/or C-39. The immunosuppressive activity of the resulting analogs was established in the mixed lympho...