Anther and pollen development were studied in Olyra humilis Nees, Sucrea monophylla Soderstr, (Bambusoideae), Axonopus aureus P. Beauv., Paspalum polyphyllum Nees ex Trin. (Panicoideae), Eragrostis solida Nees, and Chloris elata Desv. (Chloridoideae). The objective of this study was to characterise, embryologically, these species of subfamilies which are considered basal, intermediate and deriv...

Microsporogenesis has been examined in wild-type Arabidopsis thaliana and the nuclear male-sterile mutant BM3 by cytochemical staining. The mutant lacks adenine phosphoribosyltransferase, an enzyme of the purine salvage pathway that converts adenine to AMP. Pollen development in the mutant began to diverge from wild type just after meiosis, as the tetrads of microspores were released from their...

Double haploid technique is not routinely used in legume breeding programs, though recent publications report haploid plants via anther culture in chickpea (Cicer arietinum L.). The focus of this study was to develop an efficient and reproducible protocol for the production of double haploids with the application of multiple stress pre-treatments such as centrifugation and osmotic shock for gen...

Polyploidy is a prominent and significant force in plant evolution, taking place since ancient times and continuing until today. Recent cytogenetic studies in the genus Brachiaria using germplasm collected from wild African savannas in the 1980s revealed that most species and accessions within species are polyploid. Diploid, tetraploid, and pentaploid accessions have been found. We found asynch...

High temperature can induce the production of 2n gametes and aborted pollen during microsporogenesis in Populus canescens. However, mechanism by which high induces abortion remains unknown. Here, was induced exposing male flower buds P. canescens to 38 41 °C; morphology, meiotic abnormalities, defects microtubular cytoskeleton, tapetum development were characterized. We found that dominant stag...

The analysis of microsporogenesis in endogamous plants of popcorn (S5 to S7) showed several and distinct interchanges which involve the nucleolus organizer (chromosome 6). The detection of cells with interchanges was facilitated by the presence of two nucleoli of different sizes in contrast to normal ones with a single big nucleolus. Interchange points do not always seem to be at the same place...