We apply the techniques of digital holography to obtain microscopic three-dimensional images of biological cells. The optical system is capable of microscopic holography with diffraction-limited resolution by projecting a magnified image of a microscopic hologram plane onto a CCD plane. Two-wavelength phase-imaging digital holography is applied to produce unwrapped phase images of biological ce...

This paper presents an IF1F2 color space for describing colors in microscopic images. Unlike the classical color spaces that treat the R, G, and B (RGB) components of a pixel’s color as independent elements, the proposed color space treats RGB as a sampled spectral signal, called a RGB signal. Then, based on the Fourier spectrum analysis of the RGB signal, the 1-D discrete Fourier transform is ...

Microscopic image analysis is an important step in biological study. Biologists study the movements of cells under certain drug treatments through a sequence of time-lapse microscopic images to determine the effect of the treatments. The development of modern bright-field microscopes allows more detailed investigations of the cell activities. However, it also brings challenges for automatic cel...