The stone crayfish Austropotamobius torrentium is a native European freshwater species with significant population declines caused by anthropogenic pressure onto its habitats, climate change and spreading of non-native invasive their pathogens. Large-scale DNA barcoding based on sequencing short fragment the mitochondrial cytochrome c oxidase subunit I ( COI ) gene revealed this represents high...

The dioecious plant Silene latifolia possesses evolutionarily young sex chromosomes, and so serves as a model system to study the early stages of sex chromosome evolution. Sex chromosomes often differ distinctly from autosomes in both their structure and their patterns of evolution. The S. latifolia Y chromosome is particularly unique owing to its large size, which contrasts with the size of sm...

A panel of microsatellites mapped to the Leishmania genome might make it possible to find associations between specific loci and phenotypic traits. To identify such loci, a Perl programme was written that scans the sequence of a genome and writes all loci containing microsatellites to a MySQL database. The programme was applied to the sequences of the L. braziliensis, L. infantum and L. major g...

PURPOSE Although mitochondrial DNA mutations have been described recently in many different tumor types, the nature and timing of such alterations remain unclear. In an effort to further examine the role of mitochondrial DNA mutations in carcinogenesis, we examined 137 premalignant lesions of the head and neck from 93 patients for DNA alterations in the poly-cytosine tract (C-tract) of the disp...

Computer science is a subject, which deals with the manipulation of data so that new data, implicit in the original, appear in a useful form. We have used the analogy of genome analysis and VIRUS (vital information recourse under siege) and analyzed MLH1, MSH2 and MSH6 gene which play an important role in repairing mistakes made in DNA replication in colon cancer. If the MLH1, MSH2, MSH6 protei...

Although a recent study of white spruce using chloroplast DNA uncovered the presence of a glacial refuge in Alaska, chloroplast failed to provide information on the number or specific localities of refugia. Recent studies have demonstrated the utility of nuclear microsatellites to refine insights into postglacial histories. The greater relative rate of mutation may allow finer scale resolution ...

MOTIVATION Resolving tandemly repeated genomic sequences is a necessary step in improving our understanding of the human genome. Short tandem repeats (TRs), or microsatellites, are often used as molecular markers in genetics, and clinically, variation in microsatellites can lead to genetic disorders like Huntington's diseases. Accurately resolving repeats, and in particular TRs, remains a chall...

The genus Eucalyptus encompasses several species of industrial importance. Many of these species have been subjected to genetic characterization using different kinds of DNA markers. More than 1000 microsatellites have been identifi ed from the genome of eucalypts and they are highly amenable for cross species transferability. During cross amplifi cation of microsatellites, homoplasy is reporte...