MicroRNAs are small RNAs with ability to attach the large number of RNA that regulate gene expression on post-transcriptional level via inhibition or degradation specific mRNAs. MiRNAs in cells primary regulators functions such as cell growth, differentiation, and apoptosis considerably influence function. The levels microRNAs change human diseases, including cancer. These changes highlight the...

Cell-free microRNAs in plasma and serum have become a promising source of biomarkers for various diseases. Despite rapid progress in this field, there remains a lack of consensus regarding optimal quantification methods, reference genes, and quality control of samples. Recent studies have shown that hemolysis occurring during blood collection has substantial impact on the microRNA content in pl...

Within the last two decades, the incidence of invasive fungal infections has been significantly increased. They are characterized by high mortality rates and are often caused by Candida albicans and Aspergillus fumigatus. The increasing number of infections underlines the necessity for additional anti-fungal therapies, which require extended knowledge of gene regulations during fungal infection...

Tiny non-coding RNAs called microRNAs (miRNAs) are crucial for cell survival and differentiation. Numerous cancer varieties, including acute myeloid leukemia, have been linked to abnormal miRNA expression (AML). Objective:

Colorectal cancer (CRC) is one of the leading malignant cancers with a rapid increase in incidence and mortality. The recurrences of CRC after curative resection are sometimes unavoidable and often take place within the first year after surgery. MicroRNAs may serve as biomarkers to predict early recurrence of CRC, but identifying them from over 1,400 known human microRNAs is challenging and cos...

BACKGROUND The preservation of microRNAs in formalin-fixed and paraffin-embedded (FFPE) tissue makes them particularly useful for biomarker studies. The utility of small RNA sequencing for microRNA expression profiling of FFPE samples has yet to be determined. METHODS Total RNA was extracted from de-paraffinized and proteinase K-treated FFPE specimens (15-20 years old) of 8 human lung adenoca...

RATIONALE Despite a recent decline of in-hospital mortality attributable to acute myocardial infarction (AMI), the incidence of ischemic heart failure (HF) in post-AMI patients is increasing. Although various microRNAs have been proposed as diagnostic indicators for AMI, no microRNAs have been established as predictors of ischemic HF that develops after AMI. OBJECTIVE We attempted to identify...

Cardiovascular disease has become the main factor of death and birth defects in the world. There are some therapeutic structures and drugs for curative and palliative therapy of the disease, but to the aim of accessing reliable therapy or to postpone onset of disease, especially for individuals with heritable coronary artery disease in their pedigree Genetic engineering technologies are making...

It has been hypothesized based on accumulated data that a class of small noncoding RNAs, termed microRNAs, are key factors in intercellular communication. Here, microRNAs present in interstitial breast tumor fluids have been analyzed to identify relevant markers for a diagnosis of breast cancer and to elucidate the cross-talk that exists among cells in a tumor microenvironment. Matched tumor in...

MicroRNAs are nature’s dimmer switches for protein translation. When expressed in sufficient abundance, these singlestranded small RNAs bind to complementary nucleotide sequences in the 3' untranslated regions of target mRNAs and direct them to RNA-induced silencing complexes (RISCs) for translational suppression and degradation. Although much has been learned about the molecular mechanisms of ...