Response surface methodology (RSM) was used to optimize the cultivation conditions for the production of phytase by recombinant Escherichia coli DH5α. The optimum predicted cultivation conditions for phytase production were at 3 hours seed age, a 2.5% inoculum level, an L-arabinose concentration of 0.20%, a cell concentration of 0.3 (as measured at 600 nm) and 17 hours post-induction time with ...

Role of disulfide bridges in phytase's unfolding-refolding was probed using dynamic light scattering. Phytase was unfolded by guanidinium chloride and then refolded by removing the denaturant by dialysis. Thiol reagents prevented refolding; thus, disulfide bridge formation is an integral step in phytase folding. Catalytic demise of phytase after unfolding and refolding in presence of Tris(2-car...

A study was conducted to investigate the effects of thermostable phytase supplementation on the growth performance and nutrient digestibility of broilers for 42 d. 1-day-old Arbor Acres chicks with similar initial body weight were randomly divided into 3 treatments consisting 6 pens of 20 chicks per pen. The control group was given maize-soybean meal basal diet and the other two groups were fed...

Phytase expressed and anchored on the cell surface of Pichia pastoris avoids the expensive and time-consuming steps of protein purification and separation. Furthermore, yeast cells with anchored phytase can be used as a whole-cell biocatalyst. In this study, the phytase gene of Citrobacter amalonaticus was fused with the Pichia pastoris glycosylphosphatidylinositol (GPI)-anchored glycoprotein h...

Two studies were conducted to determine the true P digestibility (TPD) of black-eyed pea (BEP) and peanut flour (PNF) and the TPD response to phytase supplementation using the regression method. Sequential diets containing 115, 230, or 345 g of BEP/kg (experiment 1) and 115, 230, or 345 g of PNF/kg (experiment 2) without or with 1,000 units of phytase/kg were formulated. Chromic oxide was added...

background: degradation of phytic acid to inorganic phosphate in domestic animals’ diets requires thermostable phytase. although basillus subtilis phytase shows a potential to be degraded phytate complex in high temperature, the enzyme activities and yields need to be increased to make them possible for industrial application. methods: the phytase gene from bacillus subtilis dr8886 was isolated...

Traditional supplemental dietary phytase loses activity during steam pelleting. The thermal tolerance and bioefficacy of a phytase product with a thermoprotective coating [coated phytase (C-phytase)] was compared in mash and pelleted diets to a traditional, uncoated phytase (U-phytase) added to a negative control (NC) diet, formulated with reduced dietary Ca and P, and compared with a corn-soyb...

Both 1,25-dihidroxyvitamin D3 and phytase are known to improve phosphorus utilization in broilers(Biehl and Baker,J Nutr 1997). Solanum glaucophyllum(Sg) is a plant containing high amounts of 1,25dihydroxyvitamin D bound to a glycoside. It may be an economical source of 1,25-dihydroxyvitamin D for animal feeds. We examined the effect of Sg, with and without phytase, on phosphorus utilization in...

A study was conducted to study the effects of thermostable phytase supplementation on the growth performance and Ca and P metabolism of broilers for 21 d. 1-day-old Arbor Acres chicks with similar initial body weight were randomly divided into 3 treatments consisting 8 pens of 10 chicks per pen. The control group was given maize-soybean meal basal diet, and the other two groups were fed the bas...