The respiration rate of green capsicum cv. ‘Swarna’ fruits harvested at mature green stage were determined under closed system at 10, 15 and 20 oC temperatures. A simple Michaelis-Menten kinetic model coupled with Arrhenius-type equation, which describes temperature as a function of respiration rate, was used to model the respiration rate of capsicum. The respiration rate model parameters at de...

To determine the effect of gelatinase activity of mosquitocidal protein of P. fluorescens Migula strain (VCRC-B426) on larvae and pupae of Culex quinquefasciatus was studied. The results of Enzchek Gelatinase/Collagenase assay revealed that the mosquitocidal metabolite exhibited the gelatinase/collagenase activity and efficiently digested the fluorescein conjugate-DQ gelatin to yield highly flu...

The Michaelis-Menten equation has played a central role in our understanding of biochemical processes. It has long been understood how this equation approximates the dynamics of irreversible enzymatic reactions. However, a similar approximation in the case of networks, where the product of one reaction can act as an enzyme in another, has not been fully developed. Here we rigorously derive such...

This paper describes a microanalytical method for determining enzyme kinetics using a continuous-flow microfluidic system. The analysis is carried out by immobilizing the enzyme on microbeads, packing the microbeads into a chip-based microreactor (volume approximately 1.0 nL), and flowing the substrate over the packed bed. Data were analyzed using the Lilly-Hornby equation and compared to value...

Biological networks have been recently found to exhibit many topological properties of the so-called complex networks. It has been reported that they are, in general, both highly skewed and directed. In this paper, we report on the dynamics of a Michaelis-Menten like model when the topological features of the underlying network resemble those of real biological networks. Specifically, instead o...

We present a simple method for estimating kinetic parameters from progress curve analysis of biologically catalyzed reactions that reduce to forms analogous to the Michaelis-Menten equation. Specifically, the Lambert W function is used to obtain explicit, closed-form solutions to differential rate expressions that describe the dynamics of substrate depletion. The explicit nature of the new solu...