We have developed sparsely cross-linked "nanogels", sub-colloidal polymer structures composed of covalently linked, linear polyacrylamide chains, as novel DNA sequencing matrices for capillary electrophoresis. The presence of covalent cross-links affords nanogel matrices with enhanced network stability relative to standard, linear polyacrylamide (LPA), improving the separation of large DNA frag...

   The objective of the present study is to develop and investigate the swelling behavior of pH-sensitive Superporous Hydrogel (SPH) and SPH composite (SPHC). A novel superporous hydrogel containing poly (methacrylic acid-co-acrylamide) was synthesized from methacrylic acid and acrylamide through the aqueous solution polymerization, using N,N-methylenebisacrylamide as a crosslinker and ammonium...

: Unwanted water production from oil and gas reservoirs is a serious problem for producers. Preformed particle gel (PPG) treatment is a benefit approach to control excess water production. Swelling percentage of PPG samples in saline water is a key factor affecting the efficiency of the water conformance process. In this study, an efficient series of PPGs were synthesized and their swelling beh...

This thesis focuses on the development of new stationary phases for use in hydrophilic interaction liquid chromatography using TRIS‐based and phosphorylcholine typed monomers and porous silica particles as starting substrates. In this thesis, several ways of polymerizing highly hydrophilic mono‐ mers onto pore surfaces of silica supports are described, based on several “grafting from” schemes. ...

An integrated approach to temperature-controlled chromatography, involving copolymer modified agarose adsorbents and a novel travelling cooling zone reactor (TCZR) arrangement, is described. Sepharose CL6B was transformed into a thermoresponsive cation exchange adsorbent (thermoCEX) in four synthetic steps: (i) epichlorohydrin activation; (ii) amine capping; (iii) 4,4'-azobis(4-cyanovaleric aci...

Frontal Polymerization 1,2 (FP) is an innovative technique that converts a monomer into a polymer as a result of the propagation of a reaction front along the reactor. In this work, the above technique has been used for the first time to synthesize nanocomposites, mainly based on hydrogels. Indeed, montmorillonite (MMT)-type clays and polyhedral oligomeric silsesquioxanes (POSS) have been dispe...

Superparamagnetic iron oxide nanoparticles (SPIONs) have been widely utilized for the diagnosis and therapy of specific diseases, as magnetic resonance imaging (MRI) contrast agents and drug-delivery carriers, due to their easy transportation to targeted areas by an external magnetic field. For such biomedical applications, SPIONs must have multifunctional characteristics, including optimized s...

The factors that limit the stability of adenylate kinase and acetate kinase in solution have been examined and compared with those that determine stability under conditions encountered during photochemically initiated polymer gel formation in solutions of acrylamide and N,N'-methylene-bisacrylamide. Both adenylate kinase (from rabbit and pig muscle) and acetate kinase (from E. Coli) contain cys...

A hydrophilic chiral capillary monolithic column for enantiomer separation in CEC was prepared by coating cellulose tris(3,5-dimethylphenyl-carbamate) (CDMPC) on porous hydrophilic poly(acrylamide-co-N,N'-methylene-bisacrylamide) (poly(AA-co-MBA)) monolithic matrix with confine of a fused-silica capillary. The coating conditions were optimized to obtain a stable and reproducible chiral stationa...

Some pH responsive polymeric matrix of Linseed (Linum usitatissimum), L. hydrogel (LSH) was prepared by free radical polymerization using potassium persulfate (KPS) as an initiator, N,N-methylene bisacrylamide (MBA) as a crosslinker, acrylic acid (AA) and methacrylic acid (MAA) as monomers; while ketoprofen was used as a model drug. Different formulations of LSH-co-AA and LSH-co-MAA were formul...