An interesting observation was found during our continued studies on the hydrolysis of ibuprofen esters by Candida rugosa lipase (CRL). An important role is played by pH in the stereospecific hydrolysis of these esters. The flap region of CRL plays a significant role in the access of the substrate to the active site of the enzyme. At pH 5.6, 48% of the methyl ester and 5% of the butyl ester of ...

Polyoxometalate-bis-pyrene receptors for phenyl-C61-butyric acid methyl ester (PCBM) provide an interfacial layer (IFL) material enhancing the open circuit voltage of organic photovoltaic device about 34% under applied bias ±5 V.

The purpose of this work is to study the esterification liquid olefins with acetic acid in presence catalysts and structure properties synthesized saturated esters. 1-Hexene, 2-methyl-1-pentene, 2-ethyl-1-pentene, 3-methyl-1-pentene, 3-methyl-2-pentene were used as for reaction: H2SO4 HCl. influence various factors (the nature catalysts, relative activity temperature a) on rate reaction was stu...

Malaria still remains a life-threatening parasitic disease with universal targets set for control and elimination. This study aimed to evaluate the in vitro antimalarial susceptibility of Plasmodium falciparum isolates berghei selected agents column chromatographic subfractions Glyphaea brevis leaves extract FTIR GCMS SF8. Trager Jensen as well World Health Organisation (WHO) standardised micro...

The A and J series of prostaglandins (PGs) accumulate in the nuclei to suppress the proliferation of cancer cells. Here we report that Delta7-PGA1 methyl ester, a synthetic anti-cancer PG, increased the level of mRNA for the cyclin-dependent kinase inhibitor p21 in human leukaemia HL-60 cells. The induction of p21 was associated with the accumulation of hypophosphorylated retinoblastoma protein...

Pure rotational spectra of a prototypical prochiral ester, methyl benzoylformate (MBF), and the product of its enantioselective reduction, (R)-(-)-methyl mandelate (MM), were measured in the range of 5-16 GHz, using a cavity-based molecular beam Fourier-transform microwave spectrometer. Potential conformers were located using density functional theory calculations, and one conformer of each spe...

The secondary signals emanating from increased glucose metabolism, which lead to specific increases in proinsulin biosynthesis translation, remain elusive. It is known that signals for glucose-stimulated insulin secretion and proinsulin biosynthesis diverge downstream of glycolysis. Consequently, the mitochondrial products ATP, Krebs cycle intermediates, glutamate, and acetoacetate were investi...

In most bacteria the last step in synthesis of the pimelate moiety of biotin is cleavage of the ester bond of pimeloyl-acyl carrier protein (ACP) methyl ester. The paradigm cleavage enzyme is Escherichia coli BioH which together with the BioC methyltransferase allows synthesis of the pimelate moiety by a modified fatty acid biosynthetic pathway. Analyses of the extant bacterial genomes showed t...

Protein methylation reactions can play important roles in cell physiology. After labeling intact Saccharomyces cerevisiae cells with S-adenosyl-l-[methyl-(3)H]methionine, we identified a major methylated 49-kDa polypeptide containing [(3)H]methyl groups in two distinct types of linkages. Peptide sequence analysis of the purified methylated protein revealed that it is eukaryotic elongation facto...

The purpose of this research is the synthesis alkyl esters from CPO through transesterification reaction using catalyst CaO-MgO. This experimental laboratory stages: (1) refinement, (2) determination free fatty acid numbers, (3) activation CaO-MgO catalysts, (5) with alcohol (methanol and ethanol), (6) identification components synthesized ester GC-MS,characterization including density, viscosi...