The largest of the gene clusters coding for proteins involved in methanol oxidation is the cluster mxaFJGIR(S)ACKLDEHB. Disruption of most of these genes leads to lack of growth on methanol. The previous results showed that the mutant lacking MxaD grows on methanol although at a low rate. This is explained by the low rate of methanol oxidation by whole cells. The specific activity of methanol d...

background: herbal distillates had been used for many centuries as herbal medicine in traditional persian medicine. recently, some studies claimed the presence of methanol in different types of herbal distillates which can cause different complications or even death as methanol is highly toxic. the main purpose of this study was to determine methanol content in the commonly-used industrial herb...

The effect of pretreatment conditions on the oxidation state and activity of micelle-synthesized Pt nanoparticles supported on ZrO2 was studied for methanol decomposition and oxidation reactions. An O2-pretreatment is observed to be effective for producing clean, stable, and active nanoparticles. Pt-oxide species formed during such pretreatments were found to have little influence in methanol d...

Gastrointestinal (GI) mimic and organic solvent extracts of whole soybean powder (WSP), soy protein concentrate (SPC), and soy protein isolate (SPI) as well as soy isoflavone concentrate (SIC) were analyzed for total phenols; quinone reductase (QR) induction in hepa1c1c7 cells; antioxidant scavenging of DPPH, HOCl, ONOO(-), and O(2) (-.); and total antioxidant capacity via FRAP and ORAC assays....

When methanol, a nonsolvent, was added to dilute tetrahydrofuran solutions of an optically active polyfluorene derivative, liquid–liquid phase separation occurred, and circular dichroism (CD) was induced at a low temperature. The polymer concentration of the minor separating phase, estimated by light scattering, was very high (B0.4 g cm 3). The CD induction occurring in that concentrated phase ...

BACKGROUND Hepatitis B is a serious global public health concern. Though a safe and efficacious recombinant vaccine is available, its use in several resource-poor countries is limited by cost. We have investigated the production of Hepatitis B virus surface antigen (HBsAg) using the yeast Pichia pastoris GS115 by inserting the HBsAg gene into the alcohol oxidase 1 locus. RESULTS Large-scale p...

BACKGROUND Recent advances in intracellular staining techniques, cytometer technology, fluorescent reagents, and antibody production have expanded the number of intracellular antigens that can be analyzed by flow cytometry. Measurement of protein phosphorylation with phospho-specific antibodies has given insight into kinase signaling cascades. However, available techniques for phospho-epitope s...

Scurrula ferruginea (Jack) Danser is one of the mistletoe species belonging to Loranthaceae family, which grows on the branches of many deciduous trees in tropical countries. This study evaluated the antioxidant activities of S. ferruginea extracts. The cytotoxic activity of the selected extracts, which showed potent antioxidant activities, and high phenolic and flavonoid contents, were investi...

In the methylotrophic bacterium Methylobacterium extorquens strain AM1, MxaF, a Ca(2+)-dependent methanol dehydrogenase (MDH), is the main enzyme catalyzing methanol oxidation during growth on methanol. The genome of strain AM1 contains another MDH gene homologue, xoxF1, whose function in methanol metabolism has remained unclear. In this work, we show that XoxF1 also functions as an MDH and is ...

human alpha 1-antitrypsin (aat) cdna was obtained from hepg2 cell lines. after pcr and construction of expression vector ppiczα-aat, human aat was expressed in the yeast pichia pastoris (p.pastoris) in a secretary manner and under the control of inducible alcohol oxidase 1 (aox1) promoter. the amount of aat protein in medium was measured as 60 mg/l 72 hr after induction with methanol. results i...