Due to our increasing concerns on environmental problems and limited fossil resources, biobased production of chemicals and materials through biorefinery has been attracting much attention. Optimization of the metabolic performance of microorganisms, the key biocatalysts for the efficient production of the desired target bioproducts, has been achieved by metabolic engineering. Metabolic enginee...

Metabolic engineering was originally conceived as a systems approach to optimise biotechnologically desired traits of microbes and higher cells [1]. Microbial Cell Factories has published several review and research articles on this field over the past recent years [2-8]. Although clear breakthroughs have been achieved in the past, progress in metabolic engineering has been largely limited to i...

The biologically and commercially important terpenoids are a large and diverse class of natural products that are targets of metabolic engineering. However, in the context of metabolic engineering, the otherwise well-documented spatial subcellular arrangement of metabolic enzyme complexes has been largely overlooked. To boost production of plant sesquiterpenes in yeast, we enhanced flux in the ...

The optimization of biological systems, which is a branch of metabolic engineering, has generated a lot of industrial and academic interest for a long time. In the last decade, metabolic engineering approaches based on mathematical optimizations have been used extensively for the analysis and manipulation of metabolic networks. In practical optimization of metabolic reaction networks, designers...

Metabolic engineering is rapidly developing, with a continuous stream of technological developments being employed to expand the portfolio of molecules produced in cell factories. For chemical production (e.g., amino acids, biofuels, among others), metabolic engineering has progressed through three phases [1]. Initially, biological products were obtained through random mutagenesis of production...

The field of synthetic biology seeks to develop engineering principles for biological systems. Along these lines, synthetic biology can address human metabolic disease through the development of genetic approaches to the study and modification of metabolism. The re-engineering of natural metabolic states provides fundamental understanding of the integrated components underlying dysfunctional me...