The open pulled straw has been used effectively to vitrify preimplantation embryos because of geometricfeatures that allow rapid rates of temperature exchange. One possible inexpensive alternative to the openpulled straw are commercially-available microcapillary pipette tips commonly used for electrophoresis. Themain purpose of this study was to compare the survival rates of in vitro produced b...

In the presence of 2-mercaptoethanol peptide-(α)thioesters undergo smooth conversion to their corresponding peptide-(α)carboxylates. This general and operationally simple reaction extends the utility of a promising new strategy for cleaving resin-bound Boc/benzyl-protected peptides without the use of hydrogen fluoride.

In comparing the use of the sulphydryl compounds 2-mercaptoethanol (2ME) and dithiothreitol (DTT) a rapid one-stage technique for titrating IgG anti-A and anti-B using DTT in an antiglobulin test was tried. The results correlated well with those obtained by either of the two-stage methods using 2ME or DTT.

To the Editor: In a recent report, Keren et al. (1 ) described a case in which the Sebia semiautomated electrophoresis method failed to demonstrate proteinuria in a 50-fold concentrated urine sample despite the presence of 2 g/24 h total protein. The reason for this failure was attributed to the presence of debris associated with cellular elements that interfered with the wicking of the urine i...