1. We have investigated the effects of ethanethiol, methanethiol and dimethyl sulphide on some metabolic processes of isolated rat hepatocytes, isolated mitochondria from liver and brain and ox-heart submitochondrial particles. 2. Ethanethiol, but not dimethyl sulphide, inhibited both gluconeogenesis and ureogenesis from various substrates in rat hepatocytes, depressed cellular ATP content and ...

THE oxidation of thiol compounds by oxygen and methylene blue has, in recent years, been very extensively studied, while their oxidation by hydrogen peroxide has suffered almost complete neglect. Abderhalden and Wertheimer [1923] found that cysteine, like other mercaptans, was instantaneously oxidised by hydrogen peroxide in neutral solution, and that this oxidation was unaffected by cyanide. A...

In 1863 Swarts* (in WShler's laboratory) noticed in the secretion of the anal glands of ~ . mephitica the presence of a basic body which from its volatility and odor he referred to methylor ethyl-amine. Later, in 1879, Loew~stated that the secretion contains a basic body which smells like trimethylamine~ and no doubt has a constitution similar to that of neurin. Up to the present time, beyond t...

2,2-Bipyridine chelates 2 zinc ions of horse liver alcohol dehydrogenase with a dissociation constant of 4.0 X low4 M. The complex shows an absorption maximum at 308 rnp with a difference extinction coefficient of 1 .l X lo4 M+ cm-r per zinc ion. Because bipyridine binds the enzyme less tightly than o-phenanthroline, and because the principal absorption maximum of the difference spectrum appear...

Planning of coal hydrogenation processes, such as liquifaction and gasification, requires consideration of public health implications. Commercial plants will require coal quantities greater than or equal to 20,000 tons/day and the large size of these plants calls for careful consideration of the potential health hazards from the wastes and products of such processes. Analysis of pollution poten...

A simple acetoxymercuration reaction for introducing covalently bound mercury atoms into nucleotides is described. The 5-mercuriacetate derivatives of UTP, CTP, dUTP, and dCTP, as well as the 7-mercuriacetate derivative of 7-deazaATP, have been prepared by this procedure and tested as substrates for nucleic acid polymerases. These nucleotides, in the absence of added mercaptan, are not polymeri...

The reactivity of mercaptans with carbonyl groups has often suggested the involvement of such combinations in the enzymatic transformation of compounds containing the carbonyl function. Although the details of the interaction between protein and substrate remain a matter of controversy, there can be no denial of the intimate involvement of protein sulfhydry1 groups with substrate in at least se...

In this study, a top cover system is investigated as a control for emissions during the aftercare of new landfills and for old landfills where biogas energy production might not be profitable. Different materials were studied as landfill cover system in lab-scale columns: mechanical-biological pretreated municipal solid waste (MBP); mechanical-biological pretreated biowaste (PB); fine (PBSf) an...

Diphosphopyridine nucleotide reacts chemically with a number of reagents, including alkali (l), cyanide (2, 3), bisulfite ions (2, 3), dihydroxyacetone (4), dithionite ions (5), hydroxylamine (6), mercaptans (7), and aromatic amines (8). All of these reagents are nucleophilic agents which react on a positive center. It is generally assumed that these reactions involve the addition of the nucleo...

The chlorinated fumigants chloropicrin (trichloronitromethane) and 1,3-dichloropropene (1,3-D) are extensively used in agricultural production for the control of soilborne pests. The reaction of these two fumigants with hydrogen sulfide species (H2S and HS-) was examined in well-defined anoxic aqueous solutions. Chloropicrin underwent an extremely rapid redox reaction in the hydrogen sulfide so...