A cDNA expression library in hMOSElox derived from poly(A)+ RNA from pumpkin endosperm was screened immunologically with a polyclonal antibody raised against partially purified gibberellin (GA) 2P,3P-hydroxylase from pumpkin endosperm. A recombinant fusion protein encoded by a selected positive clone catalyzed 3 P-hydroxylation of GA15, GA24, GA25, and GA,, and of GAI2-aldehyde, GAI2, G 4 , and...

The effects of a pumpkin paste concentrate and its components on oral glucose tolerance and serum lipid levels were determined in non-obese type 2 diabetic Goto-Kakizaki (GK) rats. In the oral glucose tolerance test, the pumpkin paste concentrate-fed group maintained a lower glucose level than the control group between 15 and 60 min. The compounds considered to be effective in improving glucose...

Vidrih R., Vidakovič S., Abramovič H. (2010): Biochemical parameters and oxidative resistance to thermal treatment of refined and unrefined vegetable edible oils. Czech J. Food Sci., 28: 376–384. In human nutrition fats are physiologically important food constituents but also the components most liable to oxidative degradation. The oils included in the study were refined (sunflower, extra-sunfl...

The characteristics of patients with non-fatal road traffic injuries who received care from non-referral hospitals are described; an assessment of the difference between the characteristics of patients who received care at a referral hospital and those of patients who were treated at non-referral hospitals is made. A retrospective study, conducted in Chon Buri Province, Thailand, reviewed infor...

The pumpkin is an important kitchen garden crop with very high nutritional value and long lifetime for storage and lot of fiber that acts as one of the best preventive factors from morbid obesity, hypertension, and cardiac diseases. As pretreatment for dehydration, osmotic dehydration creates maximum possible quality in the product by transfer of water outside the tissue of the nutrient. In thi...

This article examines the problem of using natural biostimulants and creating new biologically active substances, introducing them into production, increasing medicinal properties to increase productivity pumpkin crops in Uzbekistan. In this case, moisturising cucumber seeds biostimulators before planting, spraying during growing season, mixed (seed season) methods were used. As a solution prob...

The yield of animal-pollinated crops is threatened by bee declines, but its precise sensitivity is poorly known. We therefore determined the yield dependence of Hokkaido pumpkin in Germany on insect pollination by quantifying: (i) the relationship between pollen receipt and fruit set and (ii) the cumulative pollen deposition of each pollinator group. We found that approximately 2500 pollen grai...

The objective of these studies was to assess the degree to which bean leaf beetle, Cerotoma trifurcata (Forster), will feed on cucurbits. In 2003, we documented an infestation of C. trifurcata in a commercial pumpkin field near Rosemount, MN, USA. To evaluate C. trifurcata feeding on cucurbits, we conducted laboratory no-choice and choice test feeding studies. In the laboratory, C. trifurcata f...

The chito-oligosaccharide-specific lectin from pumpkin (Cucurbita maxima) phloem exudate has been purified to homogeneity by affinity chromatography on chitin. After SDS/PAGE in the presence of 2-mercaptoethanol, the pumpkin phloem lectin yielded a single band corresponding to a molecular mass of 23.7 kDa, whereas ESI-MS (electrospray ionization MS) gave the molecular masses of the subunit as 2...

In the Directed Feedback Vertex Set (DFVS) problem, we are given a digraph D on n vertices and a positive integer k and the objective is to check whether there exists a set of vertices S of size at most k such that F = D−S is a directed acyclic digraph. In a recent paper, Mnich and van Leeuwen [STACS 2016 ] considered the kernelization complexity of DFVS with an additional restriction on F , na...