A method involving the use of isolated cholesterol ester-storage granules as substrate is described for the assay of cholesterol esterase in rabbit ovarian tissues. Activities of cholesterol esterase 100-200-fold higher than those previously reported in ovarian tissues were measured by using this method. In addition to that of cholesterol esterase, activities of cholesterol ester synthetase, ch...

In vivo toxicity of monocrotophos on key metabolites and enzymes of the protein metabolism was investigated in important tissues of the freshwater fish Clarias batrachus. Fish were exposed to 1/10 and 1/20 of LC50 concentration for 28 days. After 28 days of exposure, some fish were transferred to monocrotophos-free water and kept in the same for 21 days (recovery period) in order to study the r...

Organophosphate (OPs) compounds are widely used in intensive agriculture to improve production, protect crops and control diseases vectors. The main mechanism of toxicity of OPs is the inhibition of the Cholinesterase enzymes. In this study the acetylcholinestrase (AChE), butyrylcholinesterase (BChE) and specific acetylcholinestrase (SAChE) activities as potential biomarkers of exposure to OPs ...

Cholinesterase inhibitors are the 'first-line' agents in the treatment of Alzheimer's disease. This article presents the latest information on their pharmacokinetic properties and pharmacodynamic activity. Tacrine was the first cholinesterase inhibitor approved by regulatory agencies, followed by donepezil, rivastigmine and recently galantamine. With the exception of low doses of tacrine, the c...

Selective and nonselective cytochromes P450 (P450) chemical inhibitors and monoclonal antibodies (mAbs) are routinely used to determine the contribution of P450 enzymes involved in the biotransformation of a drug. A fluorometric assay has been established using fluorescein diacetate as a model substrate to determine the effect of some commonly used P450 inhibitors and mAbs on human liver micros...

In this work, deep eutectic solvents (DES-based menthol and cholinium chloride) the ethanol, temperature, times were selected to extract bioactive compounds from rhizome, leaves, flowers Curcuma longa L., using ultrasound-assisted extraction. Analyzes antioxidant, flavonoids, antimicrobial, chelation Fe2+, inhibition of cholinesterase's enzymes, cytotoxicity, genotoxicity in Allium cepa cells p...

background diabetes mellitus as a chronic metabolic disease occurs in patients with partial or complete deficiency of insulin secretion or disorder in action of insulin on tissue. the disease is known to provide conditions for overgrowth of candida species. candida spp. cause candidiasis by many virulence factors such as esterase, hemolysin and phospholipase. objectives this study aimed to comp...

Following the development of a histochemical method for demonstrating non-specific esterase (1) in tissue sections by enzymatic hydrolysis of Bnaphthyl acetate, the possibility was explored of developing a similar method for lipase together with convenient calorimetric methods for measuring the activity of both enzymes in blood serum. Interest in the enzymes which hydrolyze carboxylic esters st...