There is growing evidence that equine papillomavirus type 2 (EcPV2) infection etiologically associated with the development of genital squamous cell carcinoma (SCC) and precursor lesions in equids. However, precise mechanisms underlying neoplastic progression remain unknown. To allow study EcPV2-induced carcinogenesis, we aimed to establish a primary culture model EcPV2 infection. Three-dimensi...

Introduction: IgA nephropathy (IgAN) is the most common primary glomerular disease (PGD) which could progress to renal failure and characterized by aberrant immune complex deposition. Transferrin receptor1 (TFRC), an receptor, a potential RNA binding protein (RBP) regulates expression of genes positively associated with cell cycle proliferation involved in IgAN. Molecular mechanisms TFRC affect...

Mcm proteins play an essential role in eukaryotic DNA replication, but their biochemical functions are poorly understood. Recently, we reported that a DNA helicase activity is associated with an Mcm4-Mcm6-Mcm7 (Mcm4,6,7) complex, suggesting that this complex is involved in the initiation of DNA replication as a DNA-unwinding enzyme. In this study, we have expressed and isolated the mouse Mcm2, ...

Human Rad17 (hRad17) is centrally involved in the activation of cell-cycle checkpoints by genotoxic agents or replication stress. Here we identify hMCM7, a core component of the DNA replication apparatus, as a novel hRad17-interacting protein. In HeLa cells, depletion of either hRad17 or hMCM7 with small-interfering RNA suppressed ultraviolet (UV) light- or aphidicolin-induced hChk1 phosphoryla...

the last decade, micro-RNAs (miRNAs) have emerged as major regulators of cell fate. They are involved in fine-tuning gene expression in normal developing tissues and are often aberrantly expressed in different disease states, including cancer. miRNAs are 20-25 nucleotide non-coding RNAs that repress the translation and stability of a large number of target mRNAs. The study by Brett et al in the...