Peptidoglycan fragments released by Neisseria gonorrhoeae contribute to the inflammation and ciliated cell death associated with gonorrhea and pelvic inflammatory disease. However, little is known about the production and release of these fragments during bacterial growth. Previous studies demonstrated that one lytic transglycosylase, LtgA, was responsible for the production of approximately ha...

Dioxygen activations constitute one of core issues in copper-dependent metalloenzymes. Upon O2 activation, metalloenzymes such as particulate methane monooxygenases (pMMOs), lytic polysaccharide (LPMOs) and binuclear copper enzymes PHM DβM, are able to perform various challenging C–H bond activations. Meanwhile, copper-oxygen containing complexes have been synthetized mimic the active species a...

Root-knot nematode, Meloidogyne incognita (Kofoid and White) is one of the most destructive plant parasitic nematode species in almost all agriculture horticulture crops Sri Lanka. Biologically sound controlling measures against this has been attempted from long past with ambiguous efficacy. This paper highlights nemato-toxic potential crude leaf extract Annona glabra, extracellular metabolites...

Isolation of DNA for PCR is time-consuming and involves many reagents. The aim of this work was to optimise a rapid and easy PCR methodology without previous DNA isolation. Different strains of the phytopathogenic fungus Colletotrichum lindemuthianum were used. Protoplasts were generated using lytic enzymes under high incubation temperatures using different methodologies to obtain the template....

The beta-hexosaminidase inhibitor and structural analog of the putative oxazolium reaction intermediate of lytic transglycosylases, N-acetylglucosamine thiazoline (NAG-thiazoline), was synthesized in 46% overall yield and tested as an inhibitor of Escherichia coli growth. NAG-thiazoline, at concentrations up to 1 mg/ml, was not found to affect the viability of E. coli DH5alpha. However, the com...

β-lactam antibiotics inhibit bacterial cell wall assembly and, under classical microbiological culture conditions that are generally hypotonic, induce explosive cell death. Here, we show that under more physiological, osmoprotective conditions, for various Gram-positive bacteria, lysis is delayed or abolished, apparently because inhibition of class A penicillin-binding protein leads to a block ...