This report shows that, of the Tween series (Tween 20, Tween 40, Tween 60 and Tween 80) of nonionic surfactants adsorbed on gold nanoparticles (NPs), Tween 80 makes the NPs most stably dispersed in aqueous solutions with or without the presence of representative biological molecules, such as nucleic acids or proteins of different sizes, isoelectric points (pIs) and shapes. In addition, the stab...

Inactivated Hemagglutinating Virus of Japan Envelope (HVJ-E) was immobilized on electrospun nanofibers of poly(ε-caprolactone) by layer-by-layer (LbL) assembly technique. The precursor LbL film was first constructed with poly-L-lysine and alginic acid via electrostatic interaction. Then the HVJ-E particles were immobilized on the cationic PLL outermost surface. The HVJ-E adsorption was confirme...

Methods to manipulate and visualize isolated DNA and oligonucleotide strands are important for investigation of their biophysics as well as their interactions with proteins. Herein, we report such a method by combining a block copolymer surface functionalization strategy with microfluidics. The copolymer poly(l-lysine-graft-polyethylene glycol) (PLL-g-PEG) coated one surface of the microfluidic...

Ultrathin polypeptide multilayer films are assembled by the sequential electrostatic adsorption of monolayers of poly-l-lysine and poly-l-glutamic acid onto carboxylic acid terminated alkanethiol-modified gold surfaces. The polypeptide multilayer films are hydrophilic, can incorporate electroactive species such as ferri/ferrocyanide, and are stable when immersed in organic solvents such as 1,2-...

We report herein a new and enabling approach for decorating both abiotic and cell surfaces with the extracellular matrix IKVAV peptide in a site-specific manner using strain promoted azide-alkyne cycloaddition. A cyclooctyne-derivatized IKVAV peptide was synthesized and immobilized on the surface of pancreatic islets through strain-promoted azide-alkyne cycloaddition with cell surface azides ge...

Ultrathin (<20 nm) polypeptide multilayer films are assembled by the electrostatic adsorption of alternating monolayers of poly(L-lysine) and poly(L-glutamic acid) onto carboxylic acid terminated alkanethiol-modified gold surfaces. These polypeptide multilayer films are hydrophilic, can bind electroactive anions such as ferri/ ferrocyanide, and are stable when immersed in organic solvents such ...

glucose 6- phosphate dehydrogenase from streptomyces aureofaciens was purified andinactivated by pyridoxal 5′-phosphate (plp). the inactivation was a pseudo-first order and time-dependentreaction. complete inactivation was achieved at 0.2mm plp within 16 minutes. the type of inhibition wascompetitive with respect to glucose 6- phosphate. spectral characteristics of plp-enzyme complexcorresponde...

the main objective of this study was to elucidate the effect of short- term exposure of japanese quail eggs to acute high incubation temperature on embryonic development and on the ability of post- hatched chicks to cope with subsequent heat stress conditions during the growing period. a total of 998 japanese quail eggs were divided into two groups, the first group (470 eggs) was maintained at ...

the aim of this study was to evaluate the effects of balancing low protein diets for methionine and lysine on performance of early lactation holstein cows under hot environmental temperature. twenty one multiparous holstein cows in early lactation were allocated to three experimental rations including, 1) high protein ration with 17.5% cp, 2) medium protein ration with 16% cp and 12 g/d rumen p...

Smectite clays are hydrated layer silicates that, like micas, occur naturally in abundance. Importantly, they have readily modifiable interlayer spaces that provide excellent sites for nanochemistry. Vermiculite is one such smectite clay and in the presence of small chain-length alkyl-NH(3)Cl ions forms sensitive, 1-D ordered model clay systems with expandable nano-pore inter-layer regions. The...