The effects of glass transition of, and protein conformation in, the dried solid on the storage stability of freeze-dried recombinant human interleukin-1 receptor antagonist (rhIL-1ra) were examined. Glass transition is a temperature-dependent phenomenon. Amorphous materials become hard and brittle at temperatures below their characteristic glass transition temperatures (Tg) such that diffusion...

In this work, a blend of gelatin A (GA) and polyvinylpyrrolidone (PVP K30) was used for semi-solid 3D printing bone scaffold ibuprofen (IBU) delivery. The cross-linking the obtained performed with 1% glutaraldehyde (GTA) solution, followed by lyophilization. thermal mechanical properties, as well drug release profiles, kinetics prepared scaffolds were investigated. cross-linked lyophilized has ...

Abstract Purpose Uridine diphosphate glucose pyrophosphorylase (UGPase) plays an important role in metabolism, catalyzing the reversible formation and decomposition of UDP-glucose (UDPG). In previous work, we found that UGPase is a key enzyme lyophilization response for Lactobacillus acidophilus ( L. ). However, its function regulatory mechanism freeze-drying stress are unknown. Herein, effect ...